Microarray Analysis of Gene Expression Alteration in Human Middle Ear Epithelial Cells Induced by Asian Sand Dust.
- Author:
Yoon Young GO
1
;
Moo Kyun PARK
;
Jee Young KWON
;
Young Rok SEO
;
Sung Won CHAE
;
Jae Jun SONG
Author Information
- Publication Type:Original Article
- Keywords: Particulate Matter; Asian Sand Dust; Air Pollution; Microarray Analysis; Otitis Media
- MeSH: Air Pollution; Apoptosis; Asian Continental Ancestry Group*; Cell Cycle; Cell Differentiation; Cell Proliferation; Dust*; Ear, Middle*; Epithelial Cells*; Gene Expression*; Humans*; Microarray Analysis*; Otitis Media; Particulate Matter; Real-Time Polymerase Chain Reaction; RNA; Silicon Dioxide*; Transcriptome
- From:Clinical and Experimental Otorhinolaryngology 2015;8(4):345-353
- CountryRepublic of Korea
- Language:English
- Abstract: OBJECTIVES: The primary aim of this study is to evaluate the gene expression profile of Asian sand dust (ASD)-treated human middle ear epithelial cell (HMEEC) using microarray analysis. METHODS: The HMEEC was treated with ASD (400 microg/mL) and total RNA was extracted for microarray analysis. Molecular pathways among differentially expressed genes were further analyzed. For selected genes, the changes in gene expression were confirmed by real-time polymerase chain reaction. RESULTS: A total of 1,274 genes were differentially expressed by ASD. Among them, 1,138 genes were 2 folds up-regulated, whereas 136 genes were 2 folds down-regulated. Up-regulated genes were mainly involved in cellular processes, including apoptosis, cell differentiation, and cell proliferation. Down-regulated genes affected cellular processes, including apoptosis, cell cycle, cell differentiation, and cell proliferation. The 10 genes including ADM, CCL5, EDN1, EGR1, FOS, GHRL, JUN, SOCS3, TNF, and TNFSF10 were identified as main modulators in up-regulated genes. A total of 11 genes including CSF3, DKK1, FOSL1, FST, TERT, MMP13, PTHLH, SPRY2, TGFBR2, THBS1, and TIMP1 acted as main components of pathway associated with 2-fold down regulated genes. CONCLUSION: We identified the differentially expressed genes in ASD-treated HMEEC. Our work indicates that air pollutant like ASD, may play an important role in the pathogenesis of otitis media.
