miRNA-296-5p functions as a potential tumor suppressor in human osteosarcoma by targeting SND1.
10.1097/CM9.0000000000001400
- Author:
Ya-Zeng HUANG
1
;
Jun ZHANG
2
;
Jian-Jian SHEN
3
;
Ting-Xiao ZHAO
2
;
You-Jia XU
1
Author Information
1. Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Soochow, Jiangsu 215004, China.
2. Department of Orthopedics, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 315000, China.
3. Department of Orthopedics, Cixi People's Hospital, Ningbo, Zhejiang 310014, China.
- Publication Type:Journal Article
- MeSH:
Bone Neoplasms/genetics*;
Cell Line, Tumor;
Cell Movement/genetics*;
Cell Proliferation/genetics*;
Endonucleases/genetics*;
Gene Expression Regulation, Neoplastic;
Genes, Tumor Suppressor;
Humans;
MicroRNAs/genetics*;
Osteosarcoma/genetics*
- From:
Chinese Medical Journal
2021;134(5):564-572
- CountryChina
- Language:English
-
Abstract:
BACKGROUND:The pathogenesis of osteosarcoma (OS) is still unclear, and it is still necessary to find new targets and drugs for anti-OS. This study aimed to investigate the role and mechanism of the anti-OS effects of miR-296-5p.
METHODS:We measured the expression of miR-296-5p in human OS cell lines and tissues. The effect of miR-296-5p and its target gene staphylococcal nuclease and tudor domain containing 1 on proliferation, migration, and invasion of human OS lines was examined. The Student's t test was used for statistical analysis.
RESULTS:We found that microRNA (miR)-296-5p was significantly downregulated in OS cell lines and tissues (control vs. OS, 1.802 ± 0.313 vs. 0.618 ± 0.235, t = 6.402, P < 0.01). Overexpression of miR-296-5p suppressed proliferation, migration, and invasion of OA cells. SND1 was identified as a target of miR-296-5p by bioinformatic analysis and dual-luciferase reporter assay. Overexpression of SND1 abrogated the effects induced by miR-296-5p upregulation (miRNA-296-5p vs. miRNA-296-5p + SND1, 0.294 ± 0.159 vs. 2.300 ± 0.277, t = 12.68, P = 0.003).
CONCLUSION:Our study indicates that miR-296-5p may function as a tumor suppressor by targeting SND1 in OS.
