Serum microRNA expression profiling revealing potential diagnostic biomarkers for lung adenocarcinoma.
10.1097/CM9.0000000000001100
- Author:
Xia SHAN
1
;
Lan ZHANG
1
;
Dan-Xia ZHU
2
;
Xin ZHOU
1
;
Huo ZHANG
1
;
Qing-Xie LIU
1
;
Jian-Wei TANG
3
;
Wei WEN
3
;
Tong-Shan WANG
1
;
Wei ZHU
1
;
Ping LIU
1
Author Information
1. Department of Oncology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
2. Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213003, China.
3. Department of Thoracic Surgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
- Publication Type:Journal Article
- MeSH:
Adenocarcinoma of Lung/genetics*;
Biomarkers;
Biomarkers, Tumor/genetics*;
Gene Expression Profiling;
Humans;
Lung Neoplasms/genetics*;
MicroRNAs/genetics*;
ROC Curve
- From:
Chinese Medical Journal
2020;133(21):2532-2542
- CountryChina
- Language:English
-
Abstract:
BACKGROUND:Recent studies have demonstrated that microRNAs (miRNAs) in the blood circulation can serve as promising diagnostic markers for cancers. This four-stage study aimed at finding serum miRNAs as potential biomarkers for lung adenocarcinoma (LA) diagnosis.
METHODS:The study was carried out between 2016 and 2017. The Exiqon miRNA qPCR panel (3 LA vs. 1 normal control [NC] pooled serum samples) was used for initial screening to acquire miRNA profiles. Thirty-five dysregulated miRNAs were further evaluated in the training (24 LA vs. 24 NCs) and testing stages (110 LA vs. 110 NCs) using quantitative real-time polymerase chain reaction assays.
RESULTS:Four serum miRNAs (miR-133a-3p, miR-584-5p, miR-10b-5p, and miR-221-3p) were significantly overexpressed in LA patients compared with NCs. The diagnostic value of the four-miRNA panel was validated by an external cohort (36 LA vs. 36 NCs). The areas under the receiver operating characteristic curve of the four-miRNA panel in the training, testing, and external validation stages were 0.734, 0.803, and 0.894 respectively. Meanwhile, the expression level of miR-221-3p was much higher in LA tumor samples than that in the adjacent normal tissues (19 LA vs. 19 NCs). The expression level of miR-10b-5p was also elevated in the serum-derived exosomes samples (18 LA vs. 18 NCs). The expression of miR-133a-3p, miR-584-5p, and miR-10b-5p was significantly elevated in LA patients with epidermal growth factor receptor mutation compared with NCs.
CONCLUSION:The study established a four-miRNA signature in serum that could improve the diagnostic capability of LA.
