Effect of Horse Oil on Anti-bacterial, Inflammatory Cytokines, and Type I Collagen Expressions in Human HaCaT Keratinocytes and Fibroblasts.
- Author:
Kyoung Hwa CHOI
1
;
Young Sun LEE
;
Ji Hyun YOON
;
Wang Keun YOO
;
Mi Ryeo KIM
;
Kyu Suk LEE
;
Jae We CHO
Author Information
1. Department of Dermatology, Keimyung University School of Medicine, Daegu, Korea. janylove99@dsmc.or.kr
- Publication Type:Original Article
- Keywords:
Anti-bactericidal effect;
Horse oil;
IL-10;
Type I collagen
- MeSH:
Blotting, Western;
Collagen;
Collagen Type I*;
Cytokines*;
Diffusion;
Fibroblasts*;
Horses*;
Humans*;
Interleukin-10;
Keratinocytes*;
Real-Time Polymerase Chain Reaction;
Skin;
Up-Regulation
- From:Korean Journal of Dermatology
2014;52(1):1-6
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Anti-inflammation, anti-bactericidal, and collagen synthesis are important for health skin conditions. However, the effect of horse oil on anti-inflammation, anti-bactericidal, and collagen synthesis is largely unknown. OBJECTIVE: The aim of this study was to evaluate the anti-bactericidal, anti-inflammatory, and synthesis of type I collagen of horse oil. METHODS: Anti-bacterial effect was evaluated by disc diffusion test. Expressions of inflammatory cytokines were studied by RT-PCR analysis, real time PCR. Type I collagen expression was evaluated by Western blot in human HaCaT kertinocytes and fibroblasts. RESULTS: Our data showed that horse oil exerted anti-bacterial effect on P.acnes and S.aureus. Expression of IL-10 was increased by horse oil-treated HaCaT cells. In addition, increased expression of type I collagen was observed in horse oil-treated human skin fibroblasts. CONCLUSION: Horse oil exerts an anti-bactericidal effect against P.acnes and S.aureus. In addition, anti-inflammatory and anti-aging effects of horse oil will be mediated by up-regulation of IL-10 and type I collagen, respectively.
