Effect of moxibustion on expression of GAP-43 in sciatic nerve trunk and ventral horn of spinal cord of rats with primary sciatica and its nerve repair function.
10.13703/j.0255-2930.20191207-0005
- Author:
Meng-Meng WU
1
;
Li-da ZHANG
2
;
Kun YANG
1
;
Xing-Zi LUO
2
;
Jian-Feng TU
3
;
Sheng-Chao CAI
1
Author Information
1. Department of Geriatrics, Second Affiliated Hospital of Anhui University of CM, Hefei 230000, China.
2. Graduate School of Anhui University of CM.
3. Department of Acupuncture and Moxibustion, Beijing Hospital of TCM, Capital Medical University.
- Publication Type:Journal Article
- Keywords:
Point GB 30 (Huantiao);
growth-associated protein-43 (GAP-43);
moxibustion;
nerve repair;
primary sciatica
- MeSH:
Animals;
Electroacupuncture;
GAP-43 Protein/genetics*;
Male;
Moxibustion;
Rats;
Rats, Sprague-Dawley;
Sciatic Nerve;
Sciatica/therapy*;
Spinal Cord
- From:
Chinese Acupuncture & Moxibustion
2021;41(2):183-188
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of moxibustion at "Huantiao" (GB 30) on the expression of growth-associated protein-43 (GAP-43) in the sciatic nerve trunk and ventral horn of spinal cord (L
METHODS:A total of 48 healthy male SD rats were randomly divided into a normal group, a sham operation group, a model group and a moxibustion group, 12 rats in each group. The rat model of primary sciatic pain was established by chronic constriction injury (CCI) of the sciatic nerve in the model group and the moxibustion group. On the 8th day of the experiment, moxibustion was adopted at "Huantiao" (GB 30) in the moxibustion group for 5-10 min, once a day for 14 consecutive days. Sciatic nerve function index (SFI) was measured and compared in each group at day 1, 7, 14 and 21. On the 21st day of the experiment, HE staining was used to observe the morphology of ventral horn of rat spinal cord and sciatic nerve trunk. Immunohistochemical method and real-time PCR were used to detect mRNA and protein expressions of GAP-43 in the spinal cord and sciatic nerve trunk of rats.
RESULTS:On day 7, 14 and 21, there was no statistical difference in SFI between the sham operation group and the normal group (
CONCLUSION:Moxibustion at "Huantiao" (GB 30) could improve the sciatic nerve function in rats with primary sciatica and its mechanism may be related to improving the expression of GAP-43 and enhancing the self-repair ability of the sciatic nerve after injury.
