Effect of acupoint thread-embedding on macrophage polarization of epididymis adipose tissue in obese mice.
10.13703/j.0255-2930.20191114-0003
- Author:
Ling-Li LI
1
;
Jin-Qing HU
1
;
Si-Yi ZHANG
2
;
Yong-Hua XIE
1
Author Information
1. Department of TCM, Fourth Hospital of Wuhan City/Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430033, Hubei Province, China.
2. College of Clinical Chinese Medicine, Hubei University of CM.
- Publication Type:Journal Article
- Keywords:
acupoint thread-embedding;
epididymal fat;
macrophage polarization;
obesity
- MeSH:
Acupuncture Points;
Adipose Tissue;
Animals;
Epididymis;
Macrophages;
Male;
Mice;
Mice, Inbred C57BL;
Mice, Obese
- From:
Chinese Acupuncture & Moxibustion
2021;41(2):177-182
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of acupoint thread-embedding at "Zusanli" (ST 36) and "Fenglong" (ST 40) on the macrophage polarization of epididymis adipose tissue in obese mice, and to explore the action mechanism of acupoint thread-embedding on weight control.
METHODS:Among 30 male C57BL/6 mice, 10 mice were randomly selected and fed with normal diet, and the remaining 20 mice were fed with high-fat diet to establish the obesity model. Sixteen mice with successful obesity model were randomly divided into a model group and an acupoint thread-embedding group, 8 mice in each group. Eight mice were selected from mice which were fed with normal diet as the normal group. On the next day of successful modeling, acupoint thread-embedding was performed at "Zusanli" (ST 36) and "Fenglong" (ST 40) in the acupoint thread-embedding group, once every 10 days for 4 times. The body weight was recorded at 0, 8, 16, 24, 32, 40 days into intervention; the level of glucose metabolism was compared after intervention; the level of lipid metabolism and weight of epididymal adipose tissue were compared at the end of the intervention; the mRNA expression of M1 and M2 macrophage-related cytokines interleukin-10 (IL-6), monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) were detected by real-time PCR; the mRNA and protein expression of M1 macrophage labeled inducible nitric oxide synthase (iNOS) and M2 macrophage labeled arginase-1 (Arg-1) were detected by real-time PCR and Western blot.
RESULTS:Compared with the normal group, the body weight at 0, 8, 16, 24, 32, 40 days into intervention in the model group was increased (
CONCLUSION:Acupoint thread-embedding at "Zusanli" (ST 36) and "Fenglong" (ST 40) may play a role in weight control by regulating the polarization of macrophages.
