Establishment of Two-reference Substances for Determination of Multiple Components for 8 Kinds of Ginsenosides in Panax quinquefolium Broken Pieces
- VernacularTitle:西洋参破壁饮片中8种人参皂苷类成分双标多测法的建立
- Author:
Xianhua YU
1
;
Junling LIU
2
;
Chuanshan JIN
1
;
Yazhong ZHANG
2
;
Jincai LI
3
Author Information
1. School of Pharmacy，Anhui University of TCM，Hefei 230012，China
2. Anhui Institutes for Food and Drug Control，Hefei 230051，China
3. Bozhou Vocational and Tech nical College，Anhui Bozhou 236000，China
- Publication Type:Journal Article
- Keywords:
Panax quinquefolium broken pieces;
Ginsenoside;
Two-reference substances for determination of multiple
- From:
China Pharmacy
2021;32(11):1345-1350
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To establish the m ethod for simultaneous determination of 8 kinds of ginsenosides in Panax quinquefolium broken pieces. METHODS ：HPLC-DAD method was used to determine the contents of ginsenoside Rg 1，Re，Rb1， Rc，Ro，Rb2，Rb3，Rd in P. quinquefolium broken pieces. The determination was performed on Agilent 5 TC-C18 column with mobile phase consisted of acetonitrile- 0.2％ phosphoric acid water solution （gradient elution ）at the flow rate of 1.0 mL/min. The column temperature was set at 30 ℃. The detection wavelength was set at 203 nm，and sample size was 10 μL. Ginsenoside Re and ginsenoside Rb 2 were used as control ，liner calibration with two-reference substances correction was used to predict the retention time of other 6 components，and was compared with the relative retention time method. Using ginsenoside Re as control ， above components were quantified by the relative correction factor method ，and the results were compared with the external standard method. RESULTS ：The contents of ginsenoside Rg 1，Re，Rb1，Rc，Ro，Rb2，Rb3，Rd were 10.59-12.78，2.160-2.768， 27.492-38.880，3.154-4.018，3.368-4.080，0.343-0.755，0.961-1.415，5.857-6.923 mg/g. The accuracy of two-reference substances linear correction method to predict the retention time of components was higher ，and the absolute deviation of the predicted retention time was lower than that of the relative retention time method. There was no significant difference between the relative correction factor method and the external standard method ，and relative error was ＜3％ . CONCLUSIONS ：Established two-reference substances for determination of multiple components can be used for qualitative and quantitative analysis of 8 kinds of ginsensides in P. quinquefolium broken pieces simultaneously and accurately.
