Improvement Effects of β-boswellic Acid on Hippocampal Neurons Cells Injury Induced by Oxygen-glucose Deprivation
- VernacularTitle:β-乳香酸对海马神经元细胞氧糖剥夺损伤的改善作用
- Author:
Mingming WANG
1
;
Lei WANG
1
;
Fang DOU
1
;
Weiwei LI
1
;
Aidong WEN
1
;
Jingwen WANG
1
Author Information
1. Dept. of Pharmacy，the First Affiliated Hospital of Air Force Milit ary Medical University，Xi’an 710032，China
- Publication Type:Journal Article
- Keywords:
β-boswellic acid;
Hippocampal neurons cells;
Oxygen-glucose deprivation;
Cell apoptosis
- From:
China Pharmacy
2021;32(11):1319-1324
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study the imp rovement effects of β-boswellic acid on hippocampal neurons cells injury of rats induced by oxygen-glucose deprivation. METHODS ：The hippocampal neurons cell of rats were divided into normal control group ， model group and β-boswellic acid low-concentration ，medium-concentration and high-concentration groups （1，10，100 μmol/L）. Except for normal control group ，other groups were cultured with relevant medium and given oxygen glucose deprivation to induce oxygen-glucose deprivation induced injury model. MTT assay was adopted to detect cell viability. Chemical colorimetry was used to detect LDH activity in cell culture supernatant. Hoechst-PI staining was used to detect the morphology change of cells. Flow cytometry was used to detect early apoptosis rate of cells. The expression of apoptosis-related protein （Bcl-2，Bax and cleaved caspase-3） were detected by Western blot. RESULTS ：Compared with model group ，the survival rate of cells and protein expression of Bcl- 2 were increased significantly in β-boswellic acid medium-concentration and high-concentration groups （P＜ 0.01），while LDH activity ，early apoptosis rate ，protein expression of cleaved caspase- 3 and Bax were all decreased significantly （P＜0.05 or P＜0.01）. The densely stained nuclei and fragmentation decreased significantly. CONCLUSIONS ：β-boswellic acid can relieve oxygen-glucose deprivation induced injury of hippocampal neurons cells ，the mechanism of which may be associated with down-regulating the protein expression of cleaved caspase- 3 and Bax and up-regulating the protein expression of Bcl- 2.
