Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model
- Author:
Shu Pin Yu
;
Kien Chai Ong
;
Soon Hao Tan
;
David Perera
;
Kum Thong Wong
- Publication Type:Journal Article
- Keywords:
Japanese encephalitis virus;
quasispecies;
biological clones;
limiting dilution
- From:Neurology Asia
2020;25(3):279-284
- CountryMalaysia
- Language:English
-
Abstract:
The Japanese encephalitis virus (JEV), a leading cause of encephalitis, exists as quasispecies in clinical
isolates. Using a limiting dilution method combined with immunohistochemistry to detect viral antigens,
10 biological clones were isolated and purified from a clinical JEV isolate (CNS138/9) derived from
an autopsy brain. These biological clones were tested for neurovirulence in SK-N-MC and NIE-115
neuronal cells, and a 2-week-old, footpad-infected, JE mouse model. Nine clones were found to be
neurovirulent; one clone neuroattenuated. Although further studies are needed to determine genotypic
differences, if any, in these clones, the limiting dilution purification and neurovirulence testing methods
described herein should be useful for phenotypic studies of quasispecies of neurotropic viruses in
general, and JEV and other flaviviruses in particular.
- Full text:7.2020my0043.pdf
