The effect of silencing the endoplasmic reticulum stress-related protein calnexin on the proliferation, invasion, and migration of tongue squamous cell carcinoma cells
10.12016/j.issn.2096-1456.2021.08.005
- Author:
ZHONG qijian
1
;
JIN Tingting
2
;
PENG Yu
3
;
CHEN Weixiong
2
;
LI Jinsong
3
Author Information
1. Department of Stomatology, University of Chinese Academy Sciences-Shenzhen Hospital
2. Department of Stomatology, Longgang District Central Hospital, Affliated to Guangzhou University of Traditional Chinese Medicine
3. Department of Oral and Maxillofacial Surgery, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University
- Publication Type:Journal Article
- Keywords:
endoplasmic reticulum stress;
calnexin;
tongue squamous cell carcinoma;
SCC-9 cells;
SCC-25 cells;
proliferation;
invasion;
migration;
siRNA;
therapeutic target
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2021;29(8):535-540
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of silencing the endoplasmic reticulum stress-related protein calnexin on the proliferation, invasion, and migration of tongue squamous cell carcinoma cells.
Methods :Calnexin siRNA was transfected into SCC-9 and SCC-25 tongue squamous cell carcinoma cells, and the expression of calnexin was detected by qRT-PCR. The silencing effect of calnexin siRNA was further verified by Western blotting. CCK-8 assay was applied to detect the effect of silencing calnexin on the proliferation of tongue squamous cell carcinoma cells; Transwell assay was used to detect the effect of silencing calnexin on the invasion and migration of tongue squamous cell carcinoma cells.
Results :qRT-PCR showed that calnexin siRNA could effectively downregulate the expression of calnexin. Western blot analysis further confirmed the silencing effect of calnexin siRNA on calnexin. The CCK-8 assay showed that silencing calnexin expression on the 4th and 5th days could inhibit the proliferation of tongue squamous cell carcinoma cells, and the difference was statistically significant (P < 0.01). The Transwell assay showed that knockdown of calnexin could inhibit the invasion and migration of tongue squamous cell carcinoma cells (P < 0.001).
Conclusion:Knockdown of calnexin can inhibit the proliferation, invasion, and migration of tongue squamous cell carcinoma cells.
- Full text:沉默calnexin对舌鳞状细胞癌细胞增殖、侵袭和迁移的影响.pdf
