The effect of inhibiting p38 MAPK on the expression of genes related to enamel development in mice
10.12016/j.issn.2096-1456.2021.08.004
- Author:
LUO Xiaona
1
;
LIU Xianghui
1
;
WANG Bo
2
;
LIU Xin
1
;
XIE Xiaohua
1
Author Information
1. Department of Stomatology, The Second Affiliated Hospital of Harbin Medical University
2. Department of Stomatology, Heilongjiang Second Hospital
- Publication Type:Journal Article
- Keywords:
p38 MAPK;
SB203580;
enamel;
tooth germ;
enamel epithelium;
enamel development;
signaling pathway;
runt-related transcription factor 2;
osterix;
ameloblast markers
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2021;29(8):529-534
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of p38 mitogen activated protein kinase (p38 MAPK) on the expression of genes related to enamel development in the enamel epithelium and to provide a basis for the study of the molecular mechanism of enamel development.
Methods:The p38 MAPK-specific inhibitor SB203580 dissolved in DMSO was added to the culture medium of mouse mandibular molar tooth germs in vitro as experiment group, and mouse mandibular molar tooth germs treated with the same amount of DMSO were used as control group. Western blot was used to detect the protein expression level of phosphorylated p38 (p-p38) in the enamel epithelium. Real-time PCR was used to detect the mRNA expression levels of runt-related transcription factor 2 (Runx2), osteoblast-specific transcription factor (Osx), ameloblast markers odontogenic ameloblast associated protein (ODAM), amelotin (AMTN), matrix metalloproteinase 20 (MMP20) and kallikrein 4 (KLK4) in the enamel epithelium.
Results : Western blot results showed that under the action of the inhibitor SB203580, the phosphorylation level of p38 MAPK in mouse enamel epithelium decreased, and the difference was statistically significant (P < 0.05). Real-time PCR results showed that the expression levels of the transcription factors Runx2 and Osx and the ameloblast markers ODAM, AMTN, MMP20, and KLK4 in the SB203580 group were lower than those in the control group, and the difference was statistically significant (P < 0.05).
Conclusion:The p38 MAPK signaling pathway can mediate enamel development by regulating the expression of the transcription factors Runx2 and Osx and the ameloblast markers ODAM, AMTN, MMP20 and KLK4 in the mouse enamel epithelium.
- Full text:抑制p38 MAPK对小鼠釉质发育相关基因表达的影响.pdf
