Analysis of serum and drug resistance levels between food source and human salmonella
10.3969/j.issn.1006-2483.2021.03.015
- VernacularTitle:食源与人源性沙门氏菌的血清和耐药水平差异分析
- Author:
Xujian MAO
1
;
Bowen TU
1
;
Yingang XUE
2
;
Ying ZHAO
1
;
Junhong LI
1
;
Qiang DU
1
Author Information
1. Changzhou Center for Disease Control and Prevention, Changzhou , Jiangsu 213022, China
2. School of Environmental and Safety Engineering, Changzhou University，Changzhou, Jiangsu 213003，China
- Publication Type:Journal Article
- Keywords:
Food-borne Salmonella;
Human Salmonella;
Serotype;
Quinolone resistance gene;
Multi-site sequence classification
- From:
Journal of Public Health and Preventive Medicine
2021;32(3):63-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze serotype distribution, drug resistance, quinolone resistance gene carrying status and genetic relationship of foodborne Salmonella and human Salmonella isolates in Changzhou from 2012 to 2018, to provide scientific basis for the prevention and control of Salmonella. Methods The serum type was identified by serum agglutination and liquid chip. The antibiotic sensitivity was determined by micro broth dilution method. The quinolone antibiotic resistance gene was determined by gene sequencing method. The multilocus sequence typing ( MLST ) typing was performed on quinolone-resistant Salmonella, and the genetic relationship was analyzed by BioNumerics 8.0. Results A total of 10 and 36 serotypes were detected in 46 foodborne Salmonella strains and 152 human Salmonella strains, respectively. The dominant serotypes were Indiana Salmonella and Salmonella typhimurium. Erythromycin resistance rate was the highest in both Salmonella strains, and the proportion of multidrug-resistant bacteria was 93.47 % ( 43 / 46 ) and 80.92 % ( 123 / 152 ), respectively. 38 strains of quinolone-resistant foodborne Salmonella GyrA subunit mainly occurred double mutations Asp87Asn, Ser83Phe, ParC subunit mainly occurred single mutation Ser80Arg, 119 strains of quinolone-resistant human Salmonella qnrS gene detection rate was higher, reached 68.1 % ( 81 / 119 ) ; The dominant ST types of quinolone-resistant Salmonella from two sources were ST17 and ST19, respectively. Conclusions The antibiotic sensitivity of the two Salmonella resistant strains from Changzhou was the same ; Synergistic drug resistance, but both quinolone resistance genemutations and carry inconsistent ; The ST type distribution of quinolone resistant strains isalso inconsistent, and the genetic relationship is far. It is suggested that the probability of Salmonella resistant bacteria infection caused by food transmission in our region is small, and the treatment of the two should be differentiated.
