Establishment and application of a detection method for environmental endocrine disruptors in atmospheric particulate atter
10.3969/j.issn.1006-2483.2021.03.008
- VernacularTitle:大气颗粒物（TSP）中环境内分泌干扰物检测方法的建立与应用
- Author:
Songying XIE
1
;
Yu YANG
1
;
Jie HE
1
;
Yidan WEI
1
;
Jie YU
1
;
Jie XU
1
;
Ya LUO
1
Author Information
1. School of Public Health, Zunyi Medical University, Zunyi , Guizhou 563099, China
- Publication Type:Journal Article
- Keywords:
TSP;
EDCs;
Liquid phase extraction;
HPLC-FLD
- From:
Journal of Public Health and Preventive Medicine
2021;32(3):33-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a convenient and efficient method to detect Environmental endocrine disruptors (EDCs) in atmospheric particulate matter. Methods Atmospheric total suspended particles (TSP) was sampled through glass fiber filter paper in a medium-flow TSP sampler at a flow rate of 100L/min for 2 hours. The filter paper sample was then subjected to the treatments as follows: (1) The samples were extracted with acetone and dichloromethane (3:2,v/v) from glass fiber filter paper. (2) Ultrasonic cleaning for 30 minutes. (3) Centrifugation at 2,500r/min for 20 minutes. (4) The supernatant was dried out in water baths at 55℃, and then acetonitrile was added to make the volume constant. Chromatographic condition was : HPLC-FLD ( λex=275nm，λem=312nm) . Results The content of OP, NP and BPA in the standard series showed a good linear relationship with their respective peak areas, and all the correlation coefficients were greater than 0.99. The detection limits for BPA, OP, NP were 9.80 ng/ml, 5.60 ng/ml, and 5.30 ng/ml, respectively, and the recoveries were 92.10%~127.00%, 83.90%~97.40%, and 83.70%~101.20%, respectively. The RSD for the inter-and intra-day was less than 5%. Conclusion The method was simple, rapid, and accurate, which could be used for the detection of environmental endocrine disruptors in atmospheric particulate matter.
