Detecting tin in human blood by microwave digestion-atomic fluorescence spectrometry
10.11763/j.issn.2095-2619.2016.06.021
- Author:
Le-Zhou ZHOU
1
;
Sheng FU
1
;
Ke-Ping YU
1
;
Shou-Quan GAO
1
Author Information
1. Hunan Prevention and Treatment Institute for Occupational Disease Changsha,Hunan 410007,China
- Publication Type:Journal Article
- Keywords:
Tin;
Microwave digestion;
Atomic fluorescence;
Spectrometry;
Blood
- From:
China Occupational Medicine
2016;43(06):724-727
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a method for detecting tin in human blood by microwave digestion-atomic fluorescence spectrometry. METHODS: The microwave digestion instrument was used to digest 1. 00 mL blood sample with 4. 00 mL concentrated nitric acid( mass fraction 65. 00%) and 1. 00 mL perchloric acid( mass fraction 68. 00%). After 1. 00 mL of thiocarbamide-ascorbic acid( mass fraction 10. 00%) was added,hydrochloric acid( concentration 1. 5 mol / L) was added to a total constant volume of 10. 00 mL. Then 5. 00 mL of the sample was collected and detected by atomic fluorescence spectrophotometry. RESULTS: The good linear relationship was shown in the range of 0. 00-60. 00 μg / L,and the correlation coefficient was 0. 999 5. The limit of detection was 0. 01 μg / L. The average recovery rate was 98. 80%-103. 30%. The relative standard deviation( RSD) of within-run precision was 3. 74%-5. 26%,and the RSD of betweenrun precision was 1. 23%-4. 45%. The samples can be stored for at least 7 days under the temperature of 4 ℃.CONCLUSION: This method has the advantages of low detection limit,good precision and high sensitivity,which is suitable for the determination of tin in human blood.
