Mechanism of 1,2-dichloroethane-induced toxicity in SH-SY5Y Cells
10.11763/j.issn.2095-2619.2016.06.004
- Author:
Si LI
1
;
Hong-Ling LI
;
Na ZHAO
;
Xiang-Rong SONG
;
Hao-Zhong LIU
;
Jia-Bin CHEN
;
Long-Yuan JIANG
;
Yong-Shun HANG
;
Hai-Lan WANG
Author Information
1. Department of Cardiac Surgery ICU,the First Affiliated Hospital,Sun Yat-sen University Guangzhou,Guangdong 510080,China
- Publication Type:Journal Article
- Keywords:
1,2-Dichloroethane;
SH-SY5Y cells;
Cytotoxicity;
Free radical;
Adenosine triphosphate
- From:
China Occupational Medicine
2016;43(06):652-661
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish the cell model of human neuroblastoma cell( SH-SY5Y cell) exposed to1,2-dichloroethane( 1,2-DCE) in vitro and to explore the mechanism of 1,2-DCE-induced toxicity in SH-SY5Y cells.METHODS: SH-SY5Y cells were collected in their logarithmic growth phase and cultured in complete medium that had final concentrations of 1,2-DCE in 0,10,20,30,40,50,60,70 and 80 mmol / L for 24 hours. Cell morphology was observed and cell survival rate was examined by CCK-8 assay. Using chemical colorimetric method, the activity of lactic dehydrogenase( LDH) in the cell culture supernatant,and the intracellular level of malondialdehyde( MDA),the intracellular activities of superoxide dismutase( SOD) and adenosine triphosphate( ATP) enzymes were detected. RESULTS: With the increasing exposure concentrations of 1,2-DCE,the cell density of SH-SY5Y cells gradually decreased,the synapse became shorter,the membrane ruptured,cytoplasm condensed and cytoplasmic contents overflowed increased.With the increasing concentration of 1,2-DCE,the cell survival rate decreased( P < 0. 01),the activity of LDH in the cell culture supernatant increased( P < 0. 01). These changes had a dose-effect correlation. Intracellular MDA level,and activities of SOD,Na~+-K~+-ATP enzyme,Ca~(2+)-Mg~(2+)-ATP enzyme and total ATP enzyme increased at first and then decreased. The activity of LDH in the cell culture supernatant and cell survival rate was negatively correlated( the correlation coefficient is- 0. 907,P < 0. 01). CONCLUSION: 1,2-DCE could inhibit the proliferation of SH-SY5Y cells.The mechanism may be related to the permeability change of cell membrane,cellular damage from excessive free radicals,the decrease of free radical scavenging capacity,ATP enzyme activity and calcium overloading. SH-SY5Y cells can be used as a common cell line for 1,2-DCE cytotoxicity analysis.
