Determining indium in human whole blood,serum and urine by inductively coupled plasma-mass spectrometry
10.11763/j.issn.2095-2619.2016.03.023
- Author:
Xiao-Zuo XU
1
;
Jiang-Wei YIN
1
;
Jun-Tao HE
;
Juan YI
;
Li-Kang WU
1
Author Information
1. Bao'an District Center for Disease Prevention and Control Shenzhen,Guangdong 518101,China
- Publication Type:Journal Article
- Keywords:
Inductively coupled plasma-mass spectrometry;
Indium;
Occupational exposure;
Biological sample;
Whole blood;
Serum;
Urine;
Determination
- From:
China Occupational Medicine
2016;43(03):345-349
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a methodology for determining indium in human whole blood,serum and urine by inductively coupled plasma-mass spectrometry( ICP-MS). METHODS: The whole blood,serum and urine samples were diluted 10 times in 0. 01%( mass fraction) Triton X-100 plus 0. 50%( mass fraction) nitric acid solution,and the indium level was determined by ICP-MS. Rhodium standard solution was used as the internal standard control. RESULTS: The working curve obtained from measurement of whole blood,serum and urine of normal individuals was compared to the standard curve and showed no significant difference in quantitative analysis( P > 0. 05). The linearity range of indium concentration in whole blood,serum and urine was 0. 000-20. 000 μg / L,and all the correlation coefficients were greater than 0. 999 with a detection limit of 0. 144 μg / L. The recovery rates of whole blood,serum and urine were 87. 90%-95. 92%,91. 50%-94. 20% and 90. 40%-96. 57%,respectively. The relative standard deviations( RSDs) of within-run precision were 3. 81%-7. 05%,3. 75%-5. 90% and 4. 31%-6. 62%,respectively. The RSDs of between-run precision were 2. 90%-7. 10%,3. 80%-5. 92% and 4. 16%-5. 94%,respectively. Samples could be stored for at least 14 days under the temperature of- 20 ℃. The indium in whole blood,serum and urine of workers occupationally exposed to indium( exposure group,135 person-time) and control group workers( 120 person-time) were examined. Indium was detected for 17 person-time in whole blood and serum in the exposure group with a detection rate of 1. 26%. Indium was not detected in urine samples in exposure group. It was not detected in all samples in control group. CONCLUSION: This methodology has features of simple operation,high accuracy and good precision,which is suitable for the accurate quantitative analysis of indium in biological samples.
