Inhibitory Effect and Target Prediction of Genistein on the Growth of Human Nasopharyngeal Carcinoma CNE 1 Cells
- VernacularTitle:染料木素对人鼻咽癌CNE1细胞生长的抑制作用及靶点预测
- Author:
Wendong HE
1
;
Wenqing SU
2
;
Kunhua WEI
3
,
4
;
Ling KUI
5
;
Shuo WANG
6
;
Xiaomei GONG
6
;
Xiaonan YANG
3
,
4
;
Jianhua MIAO
1
,
2
,
3
,
4
Author Information
1. Pharmacy College，Guangxi University of TCM，Nanning 530200，China
2. Pharmacy College，Guangxi Medical University，Nanning 530021，China
3. Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement，Guangxi Botanical Garden of Medicinal Plants，Nanning 530023，China
4. Guangxi Engineering Research Center of TCM Resource Intelligence Creation，Guangxi Botanical Garden of Medicinal Plants，Nanning 530023，China
5. Pharmacy College，Jiangsu University，Jiangsu Zhengjiang 212013，China
6. National Engineering Laboratory of Southwest Endangered Medicinal Resources Development，Guangxi Botanical Garden of Medicinal Plants，Nanning 530023，China
- Publication Type:Journal Article
- Keywords:
Genistein;
Human nasopharyngeal carcinoma CNE 1 cells;
High throughput sequencing;
Mutant gene p53;
PI3K/
- From:
China Pharmacy
2021;32(10):1196-1204
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study the inhibi tory effects of genistein on the growth of human nasopharyngeal carcinoma. CNE 1 cells and predict its potential target. METHODS ：CCK-8 method was used to test the effects of 0（blank control ），12.5，25，50， 100，150 µmol/L genistein on the proliferation of CNE 1 cells after treated for 24，48，72 h. Flow cytometry was carried out to detect the effects of 0（blank control ），15，30，60 µmol/L genistein on the cell cycle and ap optosis of CNE 1 cells after treated for 24 h. Scratch test was used to investigate the effects of 0（blank control ）， 10， 20， 30 µmol/L genistein on themigration ability of CNE 1 cells after treated for 24 h. High （No.18210156） throughput sequencing was conducted to discover the differential genes in CNE 1 cells after treated with 0（blankcontrol），30 µmol/L genistein for 24 h. RT-qPCR assay was adopted to verify the mRNA expression of related differential genes in above trials. RESULTS ： Compared with blank control，12.5，25，50，100，150 µmol/L genistein sho wed significant inhibitory effect on the proliferation of CNE 1 cells（P＜ 0.01），in a concentration- time-effect manner ；15，30 µmol/L genistein could arrest CNE 1 cell cycle at G 0/G1 stage（P＜0.05 or P＜ 0.01）；30，60 µmol/L could arrest CNE 1 cell cycle at G 2/M stage and promoted cell apoptosis （P＜0.05 or P＜0.01）. 10，20，30 µmol/L genistein could significantly inhibit the migration ability of CNE 1 cells（padj＜0.01）. High throughput sequencing revealed a total of 2 271 differentialgenes（P＜0.05），1 154 of which were up-regulated while 1 117 of which were down-regulated ；8 potential target genes ，including p53，p21，STC2，FGF2，CDK6，CYCLIN D ，PI3K，AKT，were screened by cell experiment. After validated by RT-qPCR assay ，mRNA expression of p53，p21，STC2，FGF2，CDK6，CYCLIN D and AKT were significantly down-regulated（P＜0.05），which consistent with the sequencing results. CONCLUSIONS ：Genistein can effectively inhibit the growth of human nasopharyngeal carcinoma CNE 1 cells，the mechanism of which may associated with inhibiting the expression of mutant gene p53，restoring the function of wild-type P 53 protein and inhibiting the activity of PI 3K/Akt pathway.
