Effects of Sinapic Acid on Improving PC 12 Cell Damage Induced by Aβ1-42 and BDNF/TrkB/ERK Signaling Pathway
- VernacularTitle:芥子酸对Aβ1-42致PC12细胞损伤的改善作用及对BDNF/TrkB/ERK信号通路的影响
- Author:
Di XUE
1
;
Yuchao LIU
2
;
Na WANG
3
;
Xuewei LIU
4
Author Information
1. School of Pharmacy，Qiqihar Medical University，Heilongjiang Qiqihar 161006，China
2. Office of Academic Research，Qiqihar Medical University，Heilongjiang Qiqihar 161006，China
3. School of Basic Medicine，Qiqihar Medical University，Heilongjiang Qiqihar 161006，China
4. School of M ental Health，Qiqihar Medical University，Heilongjiang Qiqihar 161006，China
- Publication Type:Journal Article
- Keywords:
Sinapic acid;
Alzheimer’s disease;
A β 1-42
- From:
China Pharmacy
2021;32(10):1181-1186
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study the improvement effects of sinapic acid （SA）on PC 12 cell damage induced by Aβ1-42，and to investigate its effect on brain-derived neurotrophic factor （BDNF）/tyrosine kinase B （TrkB）/extracellular signal-regulated kinase （ERK）signaling pathway. METHODS ：PC12 cells were divided into blank group ，model group ，SA low-dose and high-dose groups（50，100 μmol/L）. Except for blank group ，cell damage was induced by Aβ1-42 in other groups ；24 h after modeling ， administration groups were added with the corresponding solution and cultured for 24 h. Morphological changes of cells in each group were observed. Cell survival rate ，mRNA expression and protein level of BDNF ，protein expression of TrkB ，ERK1/2 and phosphorylated ERK 1/2（p-ERK1/2）were detected. p-ERK/ERK ratio was calculated. RESULTS ：Compared with blank group ，the model group had shorter synapses ，looser intercellular junctions ，poor adhesion ，dim cytoplasm and more granules in cytoplasm. Cell survival rate and mRNA expression and protein level of BDNF ，the relative expression of TrkB and p-ERK 1/2 protein，p-ERK/ ERK ratio were significantly decreased （P＜0.05 or P＜0.01）. Compared with model group ，in SA high-dose group the pathological changes of the cells were significantly improved ，the survival rate of the cells ，the mRNA expression and protein level of BDNF，the relative expression of TrkB and p-ERK 1/2 protein， p-ERK/ERK ratio were significantly increased （P＜0.05 or P＜ 0.01）. CONCLUSIONS：SA can i mprove PC 12 cells damage induced by Aβ1-42，the mechanism of which may be associated with activating BDNF/TrkB/ERK signaling pathway. qq.com
