Performance of a recombinase - aided amplification assay for detection of Schistosoma japonicum infections in Oncomelania hupensis
10.16250/j.32.1374.2020281
- VernacularTitle:重组酶介导的核酸等温扩增荧光法检测日本血吸虫感染性钉螺的效能评价
- Author:
Yu-Ying YE
1
;
Song ZHAO
1
;
Yan-Hong LIU
2
;
Nian-Nian BI
1
;
Xuan DONG
1
;
Chun-Rong XIONG
1
;
Hong-Ru ZHU
1
;
Feng TANG
1
;
Xin-Yao WANG
1
;
Jian-Feng ZHANG
1
;
Qing-Jie YING
2
;
Kun YANG
1
Author Information
1. National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China
2. Jiangsu Qitian Gene Technology Co., Ltd., China
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Oncomelania snail;
Recombinase-aided amplification;
Detection efficiency
- From:
Chinese Journal of Schistosomiasis Control
2021;33(2):185-188
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the efficiency of a recombinase-aided amplification (RAA) assay for the detection of Schistosoma japonicum infections in Oncomelania hupensis snails. Methods A group test was employed. Fifty Oncomelania snails were collected as a detection sample. The detection samples without infected snails were designated as negative specimens, while the detection samples that contained different numbers of infected snails were designated as positive specimens. A total of 10 negative specimens, 10 positive specimens containing 1 infected snail, 20 positive specimens containing 2 infected snails and 10 positive specimens containing 3 infected snails were assigned. Following random grouping, 40 specimens were subject to the florescent RAA assay using a blind method. The miradium shedding method served as a gold standard, and the sensitivity, specificity, Youden’s index and coincidence rate of the florescent RAA assay were estimated. In addition, 20 samples consisted of 5 negative specimens and 15 positive specimens with 1, 2 and 3 infected snails respectively were grouped randomly. The same specimens were detected using the crushing method and fluorescent RAA assay with the blind method in a paired-design manner. Then, the test results were compared and analyzed. Results Florescent RAA assay detected 29 positives in the 30 specimens containing different numbers of infected snails, with a sensitivity of 96.67%, and 8 negatives in the 10 detection specimens without infected snails, with a specificity of 80.00%, showing a Youden’s index of 0.77. The coincidence rate was 100% among 10 repeated assays for a detection specimen. In addition, there was no significant difference in the detection of infected snails between the florescent RAA assay and the crushing method (χ2 = 0, P > 0.05), and the actual coincidence rates of the florescent RAA assay and crushing method were 95.00% (19/20) and 90.00% (18/20) with the real results, respectively. Conclusion Fluorescent RAA assay has a favorable efficiency for the detection of S. japonicum infections in Oncomelania snails, which shows a potential in screening of S. japonicum-infected Oncomelania snails.
