Screening of Hepatoprotective Activity Fractions from Ixeris chinensis and Study on Its Chemical Compositions
- VernacularTitle:山苦荬保肝活性部位筛选及其化学成分研究
- Author:
Lijun SUN
1
;
Fuhou CHANG
2
;
Tuya BAI
3
;
Yuhua WANG
3
Author Information
1. College of Pharmacy，Inner Mongolia Medical University，Hohhot 010110，China
2. Research Center for New Drug Screening，Inner Mongolia Medical University，Hohhot 010110，China
3. Research Center for New Drug Safety Evaluation，Inner Mongolia Medical University，Hohhot 010110，China
- Publication Type:Journal Article
- Keywords:
Ixeris chinensis;
Hepatoprotective activity;
Active fraction screening;
Chemical components;
Identification
- From:
China Pharmacy
2021;32(9):1089-1092
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To screen the hepatoprotective active fractions from Ixeris chinensis and study its chemical constituents. METHODS：The petroleum ether，ethyl acetate，n-butanol and residual water fractions from 70％ ethanol extract of I.chinensis were extracted by systematic solvent method. Human hepatocytes HL-7702 were induced by acetaminophen to induce liver injury model. MTT method was used to detect the protective effect of the above fractions（40 μg/mL，by the dosage of crude drug）on injured cells，and the active fractions were screened. The active fractions were separated and purified by silica gel column and Sephadex column chromatography. The structure of the compounds were identified by physical and chemical properties and spectral data （hydrogen spectrum，carbon spectrum）. RESULTS：After treated with different fractions of I. chinensis，the cell survival rate of each administration group was increased significantly，compared with model group（P＜0.01），and the n-butanol and water fractions had the strongest activity （the cell survival rates were 49.3％ and 52.2％ ，respectively）. Six compoundswere isolated from n-butanol fraction and identified as sonchifolignan A（Ⅰ），apigenin-7-O-β-D-glucopyranoside methyl ester（Ⅱ），luteolin-7-O-β-D-glucuronopyranoside methyl ester （Ⅲ），luteolin-7-O-β-D-glucopyranoside （Ⅳ），apigenin-7-O-β-Dglucopyranoside（Ⅴ）and luteolin（Ⅵ）. CONCLUSIONS：The n-butanol fraction is regarded as an effective position for protecting liver，and flavonoids are the main active omponents.KEYWORDS Ixeris chinensis；Hepatoprotective activi
