Effects of Mangiferin on Glucose and Lipid Metabolism of Insulin-resistant HepG2 Cells
- VernacularTitle:芒果苷对胰岛素抵抗HepG2细胞糖脂代谢的影响
- Author:
Zilin LI
1
;
Huijie JIN
1
;
Jia FANG
1
;
Yiming LIU
1
,
2
;
Aihua LIN
3
Author Information
1. Phase Ⅰ Clinical Trial Center，Guangdong Provincial Hospital of TCM/the Second Affiliated Hospital of Guangzhou University of TCM，Guangzhou 510120，China
2. Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome，Guangdong Provincial Hospital of TCM/the Second Affiliated Hospital of Guangzhou University of TCM，Guangzhou 510120，China
3. Dept. of Pharmacy，Guangdong Provincial Hospital of TCM/Zhuhai Hospital，the Second Affiliated Hospital of Guangzhou Un iversity of TCM，Guangdong Zhuhai 519000，China
- Publication Type:Journal Article
- Keywords:
Mangiferin;
Insulin resistance;
Human HepG 2 cells;
Glucose and lipid metabolism;
AMPK signaling pathway
- From:
China Pharmacy
2021;32(9):1082-1088
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To analyze the effects of mangiferin （MGF）on glucose and lipid metabolism in insulin resistance （IR）HepG2 cells，and to explore the potential mechanism. METHODS ：Using human hepatoma HepG 2 cells as research objects ， 1 mmol/L palmitic acid and 2 mmol/L oleic acid were used to establish the IR-HepG 2 cell model. Using metformin hydrochloride as positive control ，the effects of low-concentration ，medium-concentration and high-concentration MGF （125，250，500 μmol/L）on the corrected glucose consumption ，the contents of triglyceride （TG）and total cholesterol （TC）in IR-HepG 2 cells were detected. The mRNA expression of APN ，AdipoR2，APPL1，AMPK in the upstream of AMPK signaling pathway and IRS- 1，Akt and GLUT4 in the downstream insulin signaling pathway were detected by RT-PCR. The phosphorylation level of AMPK protein was detected by Western blot assay. RESULTS ：Compared with control group ，corrected glucose consumption ，mRNA expression of APN，AdipoR2，APPL1，AMPK，IRS-1 and GLUT 4，as well as the phosphorylation level of AMPK protein were decreased significantly in model group ，while the contents of TG and TC were increased significantly （P＜0.05 or P＜0.01）. Compared with model group ， corrected glucose consumption ， mRNA expression of APN （except for MGF medium-concentration and high-concentration groups ），AdipoR2，APPL1，AMPK（except for MGF medium-concentration and high-concentration groups ）， IRS-1（except for MGF medium-concentration and high-concentration groups ），Akt（except for positive control group ），GLUT4 （except for MGF high-concentration group ）were increased significantly in administration groups ，while the contents of TG and TC were decreased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS ：Mangiferin may activate APN ，which is the upstream target of pathway ，and then regulate AMPK signaling pathway ，so as to promote glucose uptake of IR-HepG 2 cells，reduce TG and TC contents，and improve IR and abnormal glucose and lipid metabolism.
