Study on Anti-inflammatory Effect and Mechanism of Jingulian Capsule on Inflammatory Model Rats
- VernacularTitle:金骨莲胶囊对炎症模型大鼠的抗炎作用及机制研究
- Author:
Zhili FU
1
,
2
,
3
;
Jian PU
4
;
Gonglu LI
1
,
3
;
Dan TAN
4
;
Lin ZHENG
1
;
Yongjun LI
1
;
Yong HUANG
1
Author Information
1. Guizhou Provincial Key Labortary of Pharmaceutics/National Key Labortay of Efficacy and Utilization of Medicinal Plants，Guizhou Medical University，Guiyang 550004，China
2. Dept. of Pharmaceutics，Guizhou Nursing Vocational College，Guiyang 551300，China
3. College of Pharmacy，Guizhou Medical University，Guiyang 550004，China
4. Guizhou Yibai Pharmaceutical Co.，Ltd.，Guiyang 550004，China
- Publication Type:Journal Article
- Keywords:
Jingulian capsule;
Anti-inflammatory effect;
Inflammatory cytokines;
NF-κB signal pathway
- From:
China Pharmacy
2021;32(9):1077-1082
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study the anti- inflammatory effect and mechanism of Jingulian capsule on inflammatory model rats. METHODS ：Totally 48 rats were randomly divided into blank control group ，model group ，Jingulian capsule low-dose ， medium-dose and high-dose groups （0.66，1.32，2.64 g/kg），dexamethasone group （positive control ，0.945 mg/kg），with 8 rats in each group. Blank control group and model group were given constant volume of water intragastrically ，and other groups were given relevant medicine intragastrocally ，twice a day ，for consecutive 3 days. Thirty minutes after last administration ，model group and administration groups were given lipopolysaccharide （10 mg/kg）intraperitoneally to induce inflammatory model. Six hours after intraperitoneal injection ，the contents of TNF-α，IL-1β，IL-6，PGE2 in serum were detected by ELISA. The wet to dry weight （W/D）ratio of lung tissue were determined. HE staining was used to observe the pathological changes of lung tissue . RT-PCR was used to detect the mRNA expression of TNF-α，IL-6，PGE2 and IL- 1β in lung tissue. Western blot assay was used to detect the phosphorylation of NF-κB p65 protein and the expression of IκBα protein in lung tissue. RESULTS：Compared with blank ； control group ，the contents of TNF-α，IL-1β，IL-6 and PGE 2 in serum ，the W/D ratio of lung tissue ，the expression of TNF-α，IL-1β，IL-6 and PGE 2 mRNA and the phosphorylation level of NF-κB p65 protein in lung tissue of model group weresignificantly increased ，and the expression of IκBα proteinwas significantly decreased （P＜0.05 or P＜0.01）；a large number of alveolar atrophy and collapse ，alveolar wall thickening ，lung consolidation ，and a large number of inflammatory cell infiltration could be seen in lung tissue. Compared with model group ，the contents of TNF-α，IL-1β（except for low-dose group ）， IL-6 and PGE 2 in serum ，as well as the expression of TNF-α（except for high-dose group ），IL-1β，IL-6（except for low-dose ， high-dose groups ）and PGE 2 mRNA in lung tissue were decreased significantly in Jingulian capsule groups （P＜0.05 or P＜0.01）； the W/D ratio of lung tissue was decreased significantly in Jingulian high-dose group （P＜0.05 or P＜0.01）；the expression of phosphorylation level of NF-κB p65 protein in lung tissue of Jingulian medium-dose group were decreased significantly （P＜0.05 or P＜0.01），while the expression of IκBα protein was increased significantly（P＜0.05）；the alveolar structure was clear ，the alveolar wall was slightly thickened ， and a small amount of inflammatory cell infiltration was seen in lung tissue. CONCLUSIONS：Jingulian capsule has good anti-inflammatory effect on inflammatory model rats ，the mechanism of which may be related to the inhibition of NF-κB signal pathway.
