DNA barcoding identification of commercial decoctions in traditional Chinese medicine
10.16438/j.0513-4870.2020-1750
- VernacularTitle:市售中药饮片DNA条形码鉴定研究
- Author:
Tian-yi XIN
1
;
Qian LOU
1
;
Li-jun HAO
1
;
Ran-jun LI
1
,
2
;
Yu-lin LIN
1
;
Wen-jie XU
1
;
Ting-yu MA
1
;
Ye-xuan LIN
3
;
Yi-xin DONG
3
;
Cheng-yu JIANG
3
;
Jing-yuan SONG
1
,
4
Author Information
1. Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
2. School of Life and Science, Southwest Jiaotong University, Chengdu 610031, China
3. State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Department of Biochemistry, Peking Union Medical College, Beijing 100005, China
4. Engineering Research Center of Chinese Medicine Resource, Ministry of Education, Beijing 100193, China
- Publication Type:Research Article
- Keywords:
ecoction pieces;
DNA barcoding;
ITS2;
italic>psbA-trnH;
molecular identification;
market supervision
- From:
Acta Pharmaceutica Sinica
2021;56(3):879-889
- CountryChina
- Language:Chinese
-
Abstract:
Although the guiding principles for molecular identification of traditional Chinese medicines (TCM) using DNA barcoding have been recorded in the Chinese Pharmacopoeia, there is still a lack of systematic research on its application to commercial TCM decoctions. In this study, a total of 212 commercial TCM decoctions derived from different medicinal parts such as root and rhizome, fruit and seed, herb, flower, leaf, cortex, and caulis were collected to verify applicability and accuracy of the method. DNA barcodes were successfully obtained from 75.9% (161/212) of the samples, while other samples failed to be amplified due to genomic DNA degradation. Among the 161 samples, 85.7% of them were identified as recorded species in the Chinese Pharmacopoeia (2020 edition). In addition, 14 samples could be identified as species recorded in the Chinese Pharmacopoeia and their closely related species in the same genus. Morphological identification for the unconfirmed samples showed that eight were genuine species and three were adulterants, while the other three were unidentifiable due to lack of morphological characteristics. Furthermore, the DNA barcodes of seven samples accurately mapped to the sequences of adulterants. Remarkably, counterfeit products were detected in two samples. These results demonstrate that DNA barcoding is suitable for the identification of commercial TCM decoctions. The method can effectively detect adulterants and is appropriate for use throughout the industrial chain of TCM production and distribution, and by the supervisory agencies as well.
