The diversity of endophytic fungi in endangered medicinal material Daphnes Cortex as determined by high-throughput sequencing technology
10.16438/j.0513-4870.2020-1627
- VernacularTitle:基于高通量测序技术的濒危植物黄瑞香Daphne giraldii Nitsche根部内生真菌多样性研究
- Author:
Yuan HE
;
Qi-rui MU
;
Zhen-fang BAI
;
Dan JIANG
;
Guang-xi REN
;
Chun-sheng LIU
- Publication Type:Research Article
- Keywords:
high-throughput sequencing;
Daphnes Cortex;
endophytic fungi;
iversity;
endangered plant
- From:
Acta Pharmaceutica Sinica
2021;56(3):872-878
- CountryChina
- Language:Chinese
-
Abstract:
In order to provide a scientific basis for the establishment of a Daphnes Cortex medicinal material fungus library and the screening of endophytic fungi that promote the growth of Daphnes Cortex and increase the content of daphnetin, we used Illumina high-throughput testing technology to analyze 9 Daphnes Cortex samples from Gansu and Shanxi provinces. A total of 632 766 valid sequences were obtained, including 348 OTUs, 4 phyla, 20 classes, 48 orders, 108 families, 154 genera, and 208 species. The sum of the first 3 fungal genera account for more than 65% of the total abundance, with the highest reaching 98.4%. Alternaria and Phoma are the main genuses of Daphne giraldii Nitsche, and Altemaria is the dominant genus. The endophytic fungi community of Daphnes Cortex is rich in diversity, and the order of fungal diversity in different producing areas is: Gangu County > Wutai County > Tanchang County.
