Development and application of a chemical transformational method for the indirect measurement of ligustilide content in Angelica sinensis
10.16438/j.0513-4870.2020-1484
- VernacularTitle:化学转化法间接测定当归药材中药效标志物藁本内酯含量的方法研究及应用
- Author:
Peng-cheng WU
1
,
2
;
Pan ZHAO
1
;
Xing-ping LUO
3
;
Yun-jie WANG
4
;
Xiao-ning WEI
1
;
Quan HAO
4
;
Peng-wei LIU
5
;
Rui-li LIN
6
;
Lin NI
6
;
Ping-shun SONG
6
;
Jun-xi LIU
1
Author Information
1. Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China
2. University of Chinese Academy of Sciences, Beijing 100049, China
3. School of Chemical Engineering, Northwest Minzu University, Lanzhou 730030, China
4. Lanzhou Foci Pharmaceutical CO., LTD, Lanzhou 730046, China
5. Minxian Institute of Angelica sinensis of Gansu Province, Dingxi 748400, China
6. Gansu Institute for Drug Control, Lanzhou 730000, China
- Publication Type:Research Article
- Keywords:
ligustilide;
chemical conversion;
quality standard;
italic>Angelica sinensis;
liquid chromatography
- From:
Acta Pharmaceutica Sinica
2021;56(3):841-848
- CountryChina
- Language:Chinese
-
Abstract:
The poor stability of the ligustilide (LIG) makes its quantitation in Angelica sinensis (AS) difficult. This study establishes a chemical conversion method for the determination of ligustilide content in AS and proposes a national pharmacopoeia standard. Mechanical agitation and sonication of a powdered AS extract in a methanol/cyprolamine mixture facilitated the stabilization and transformation of ligustilide. Using an external reference HPLC-DAD method, the cyclopropyl-ligustilide (LIGc) content in the mixture could be determined. The content of ligustilide was greater than 1.0% based on 144 AS specimens including 68 obtained from the originally planted areas of Qinghai and Gansu Province; 55 specimens were obtained from Minxian and Weiyuan County medicine markets, and 21 specimens for which the storage period reached or exceeded 1.5 years. According to the Hong Kong Chinese materis medica standards, the content of ligustilide in AS should not be lower than 0.6%. The developed method could also be applied to the quality control of other Chinese medicinal materials (such as Ligusticum chuanxiong) or Chinese patent medicines in which ligustilide is the main component.
