Analysis of the relationship between Clostridium genus and neonatal necrotizing enterocolitis

Dongyu Zheng; Yijing Zhou; Yijing Zhou; Lubiao Yan; Shuping Han; Hong Chi; Xuan Wang; Shiqi Zhen; Zhen Tang; Yun Liu; Lei Liu; Han Zhou; Xiang Huo

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Analysis of the relationship between Clostridium genus and neonatal necrotizing enterocolitis

VernacularTitle:梭状芽孢杆菌属致新生儿坏死性小肠结肠炎关联性分析
Author: Dongyu ZHENG ¹ ; Yijing ZHOU ¹ ; Yijing ZHOU ¹ ; Lubiao YAN ^{2
,

3} ; Shuping HAN ^{2
,

3} ; Hong CHI ⁴ ; Xuan WANG ⁵ ; Shiqi ZHEN ¹ ; Zhen TANG ¹ ; Yun LIU ⁴ ; Lei LIU ⁴ ; Han ZHOU ⁵ ; Xiang HUO ¹
Author Information

1. Institute for Food Safety and Assessment.NHC Key laboratory of Enteric Pathogenic Microbiology (Jiangsu Provincial Center for Disease Control and Prevention), Nanjing 210009,China
2. Department of Pediatrics , Woman'
3. s Hospital of Nanjing Medical University, Nanjing 210004 ,China
4. Qinhuai District Centers for Disease Control and Prevention, Nanjing 210006 ,China
5. Nanjing Metropolis Centers for Disease Control and Prevention, Nanjing 210003,China
Publication Type:Journal Article
Keywords: Neonatal necrotizing enterocolitis; 16S rRNA metagenomic sequencing; Intestinal Flora; Clostridium
From: Journal of Public Health and Preventive Medicine 2021;32(2):7-11
CountryChina
Language:Chinese
Abstract: Objective To analyze the correlation between intestinal flora changes and neonatal necrotizing enterocolitis (NEC)through 16S rRNA metagenomic sequencing and bacterial culture. Methods From September 2018 to March 2019, 10 NEC cases and 6 controls were randomly selected in the neonatal ICU ward of Nanjing maternal and child health care hospital to analyze the 16S rRNA metagenomic diversity of the for intestinal flora. The fecal samples and corresponding environmental samples were corrected from 51 cases of NEC children and their case controls to isolate and culture Clostridium. Results The dispersion of samples within the case group was smaller than that of the control group, and the sample diversity was higher than that of the control group. In the isolation and culture of Clostridium, the overall detection rate of Clostridium in the case group was 43.14% (22/51), and the detection rate of Clostridium butyricum was the highest (19.61%, 10/51). There was a statistical difference between the two groups (χ²=5.85, P=0.015 58). All Clostridium strains did not carry the A, B and E type neurotoxin genes. Conclusion: Increased intestinal flora diversity, intestinal flora abundance and changes in the abundance of Clostridium may be closely related to the intestinal environment of children with NEC; Clostridium, especially Clostridium butyricum, may be related to the occurrence of NEC.