Antibacterial Efficacy and Drug-release Behavior Study of β-tricalcium Phosphate Micro-granules Against Staphylococcus aureus and Escherichia coli

Nor Hazliana Harun; Rabiatul Basria Smn Mydin; Khairul Anuar Shariff; Nur Adila Rosdi; Davamunisvari Rames

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Antibacterial Efficacy and Drug-release Behavior Study of β-tricalcium Phosphate Micro-granules Against Staphylococcus aureus and Escherichia coli

Author: Nor Hazliana Harun ¹ ; Rabiatul Basria S.M.N. Mydin ^{1
,

2} ; Khairul Anuar Shariff ³ ; Nur Adila Rosdi ^{1
,

4} ; Davamunisvari Rames ^{1
,

4}
Author Information

1. Oncological and Radiological Sciences Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, 13200 Bertam, Kepala Batas, Pulau Pinang, Malaysia&
2. Department of Biological Sciences, 14 Science Drive 4, NUS Environmental Research Institute, National University of Singapore 117543, Singapore
3. School of Materials and Mineral Resources Engineering, Engineering Campus, Universiti Sains Malaysia, 14300, Nibong Tebal, Penang, Malaysia
4. Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, 35900 Tanjong Malim, Perak, Malaysia
Publication Type:Journal Article
Keywords: Antibacterial activity, β-Tricalcium Phosphate micro-granules, β-TCP, Escherichia coli, Staphylococcus aureus
From:Malaysian Journal of Medicine and Health Sciences 2020;16(No.3, September):202-206
CountryMalaysia
Language:English
Abstract: Introduction: This study aims to investigate different residue sizes of β-tricalcium phosphate (β-TCP) micro-granules as carriers to assess antibacterial activity and drug-control release behavior of ampicillin (AMP-) and antimycotic (AMC-). Incorporation of antibiotic into the β-TCP micro-granules and it sustain release behavior could be used as alternative solution to reduce the risk of osteomyelitis and bone infections risks. Methods: Three different residue sizes (less than 300 µm, 300 µm and 600 µm) were prepared and coated with antibiotics solution (20 µg/µl of ampicillin and 100X antimycotic solution) by using two methods; dip and stream coating. After 72 h, 1.5 mL of distilled water was added to the treated (β-TCP) micro-granules at two different pH value (5.0 and 7.4). The extracted solution was further analyzed by Kirby Bauer disc diffusion test and spectrophotometer assay. Results: The solution containing AMC-(β-TCP) micro-granules with the size of 300 µm residue produced the largest inhibition zones against Escherichia coli (E. coli). All residue sizes coated with AMP- showed no antibacterial activity against both strains; Staphylococcus aureus (S. aureus) and E.coli. Additionally, the release behavior of AMC-(β-TCP) micro-granules was found not depending on the pH, but on the size of residue. Complete drug release was rapidly observed within 48 h. Conclusion: Based on this findings, it showed AMC-(β-TCP) micro-granules had an antibacterial activity against Gram-negative strain. Specifically, it can reduced the growth rate of E. coli and the rapid release behavior of AMC(β-TCP) micro-granules help in minimizing the risk-infections in early stage of implantation.
Full text:11.2020my0842.pdf