Drug resistence and NA gene characteristics of influenza virus A/H1N1(pdm09) studied in Shanghai during 2018-2019
10.19428/j.cnki.sjpm.2020.19857
- VernacularTitle:上海市2018—2019年度A/H1N1(pdm09)亚型流感病毒的耐药性及NA基因特性研究
- Author:
Xue ZHAO
1
;
Zheng TENG
;
Fang-hao FANG
;
Hui JIANG
;
Jia-bin MOU
;
Jia-jin WU
;
Chen-yan JIANG
;
Zheng-an YUAN
;
Qing-neng LIN
;
Xi ZHANG
Author Information
1. Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China
- Publication Type:Research Article
- Keywords:
influenza virus;
drug resistance;
neuraminidase;
neuraminidase inhibitors(NAIs)
- From:
Shanghai Journal of Preventive Medicine
2020;32(5):401-
- CountryChina
- Language:Chinese
-
Abstract:
Objective A/H1N1(pdm09) viruses were the dominant strains in Shanghai during 2018-2019 influenza surveillance year.This study is to provide a scientific reference for clinical drug use by investigating the susceptibility of A/H1N1(pdm09) viruses to neuraminidase inhibitors(NAIs). Methods Sixty strains of A/H1N1(pdm09) viruses were randomly selected for testing the susceptibility and drug resistance to Oseltamivir and Zanamivir by means of neutaminidase inhibition and neuraminidase (NA) gene sequencing. Results The 60 epidemic strains all proved to be susceptible to Oseltamivir and Zanamivir and the susceptibility was not observed to be decreased or remarkably decreased.In genetic sequencing, NA was not observed to present amino acid mutation at the key sites and auxiliary sites in catalytic activity, which confirmed the results of the phototypic detection of neuraminidase inhibition. Conclusion The subtype influenza viruses A/H1N1(pdm09) circulating in Shanghai during 2018-2019 surveillance year are still sensitive to NAIs, which provides a scientific reference for clinical use of drugs.However, we monitored only a number of strains and think that the work monitoring antiviral susceptibility should be continued with the wide use of the drugs.
