Simultaneous determination of 15 mycotoxins in peanuts by ultra high performance liquid chromatography-tandem mass spectrometry with QuEChERS EMR-Lipid approach and stable isotope dilution
10.19428/j.cnki.sjpm.2020.20057
- VernacularTitle:新型脱脂前处理技术联合净化－同位素稀释－超高效液相色谱－串联质谱法测定花生中15种真菌毒素
- Author:
Chen PAN
1
;
Jian YU
;
Si-lin WU
;
Long-hai JIAN
;
Rong ZHENG
;
Ke WANG
Author Information
1. Shanghai Institute for Food and Drug Control, Shanghai 201203, China
- Publication Type:Research Article
- Keywords:
QuEChERS EMR-Lipid;
isotope dilution technique;
ultra high performance liquid chromatography-tandem mass spectrometry;
mycotoxins;
peanuts
- From:
Shanghai Journal of Preventive Medicine
2020;32(5):368-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for the simultaneous determination of 15mycotoxins in peanuts by ultra high performance liquid chromatography-tandem mass spectrometry with QuEChERS EMR-Lipid approach and stable isotope dilution. Methods The samples were extracted by 2% formic acid acetonitrile-water (50 : 50, V/V) and then purified with QuEChERS EMR-Lipid approach.The mycotoxins were fully separated on a pentafluorophenyl column under a gradient elution with methonal-0.01%formic acid aqueous solution.The mycotoxins were analyzed by UPLC-MS/MS with multiple reaction monitoring (MRM) mode and quantified by isotope internal standard method. Results Fifteen mycotoxins had good linear relationship in the certain correlation ranges with the correlation coefficients all above 0.995 and the detection limits were 0.1－10 μg/kg.The mean recoveries ranged from 81.2% to 115.3% with RSD (n=6) varying from 2.1% to 10.7%. Conclusion The method is simple, highly sensitive, practical, and proves to be suitable for quantitative analysis of 15 mycotoxins in peanuts.
