Determination of polyaminopropyl biguanide in cosmetics by high performance liquid chromatography
10.19428/j.cnki.sjpm.2021.20220
- VernacularTitle:高效液相色谱法定量测定化妆品中聚氨丙基双胍
- Author:
Jing CHEN
1
;
Long-hai JIAN
1
;
Bei-ping MAO
1
;
Xiao-qian YUAN
1
;
Rong ZHENG
1
Author Information
1. Shanghai Institute of Food and Drug Control，Shanghai 201203， China
- Publication Type:Research Article
- Keywords:
high performance liquid chromatography（HPLC）;
polyaminopropyl biguanide（PAPB）;
cosmetics
- From:
Shanghai Journal of Preventive Medicine
2021;33(4):349-354
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a high performance liquid chromatographic（HPLC） quantitative method for the determination of polyaminopropyl biguanide（PAPB） in cosmetics. Methods:Different forms of cosmetic samples were prepared by ultrasonic extraction and followed by high speed centrifugation of the extraction solution. The supernatant was degreased by hexane， and then was filtered by 0.22 μm millipore filter. The continued filtrate was taken for analysis. An Agilent reversed phase column， Zorbax SB-C18（5 μm，4.6 mm×250 mm）was used with 0.02 mol/L ammonium acetate buffer （pH=4.8） ： methanol （60∶40） as the mobile phase under the condition of isocratic elution. Diode array detection method was used for PAPB determination. Qualitative and quantitative determination of PAPB was conducted in 51 batches of commercially available cosmetics. Results:The relative standard deviations （RSD） were in the range of 1.2 %-4.4 %（n=6）； the recoveries were in the range of 97.5 %-106.5 %.The method showed a good linearity within the concentration range of 5-1 000 μg/mL with correlation coefficient of 0.999 62； The detection limit was 15 mg/kg. In 51 batches of commercially available cosmetics. One batch of makeup remover showed positive resullt， which was consistent with the UV spectrum of the standard. Conclusion:We have established a HPLC method for accurate quantification of PAPB. It can be used for analyzing the cosmetics products.
