Circular RNA FBXO11 regulates the proliferation and apoptosis of gastric cancer SNU-1 cells through targeting miR-376a-3p/SNRPB axis
DOI：10.3872/j.issn.1007-385x.2021.04.009
- VernacularTitle:环状RNA FBXO11靶向miR-376a-3p/SNRPB轴调控胃癌SNU-1细胞的增殖与凋亡
- Author:
MENG Defeng
1
;
LI Changzai
1
;
WU Chuntao
1
Author Information
1. Department of Oncosurgery, the Affiliated Hospital of North China University of Science and Technology, Tangshan 063000, Hebei, China
- Publication Type:Clinical Trial
- Keywords:
circFBXO11;
miR-376a-3p;
small nuclear ribonucleoprotein polypeptides B gene (SNRPB);
gastric cancer;
SNU-1 cell;
proliferation;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
2021;28(4):370-377
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the effect of circular RNA FBXO11 (circFBXO11) regulating the miR-376a-3p/SNRPB (small nuclear ribonucleoprotein polypeptides B gene) axis on the proliferation and apoptosis of gastric cancer SNU-1 cells. Methods: Cancer and para-cancerous tissue samples from 30 patients with gastric cancer who underwent surgical resection were surgically resected in the Department of Oncosurgery, the Affiliated Hospital of North China University of Science and Technology from January 2018 to January 2019 were collected. The positive expression rate of SNRPB protein in gastric cancer tissues was detected by Immunohistochemical staining. The expression levels of circFBXO11, miR-376a-3p and SNRPB mRNA in gastric cancer tissues, gastric cancer cell lines (SNU-1, AGS and HS-746T) and gastric mucosal cell line GES1 were detected by qPCR. Dual luciferase reporter gene assay was used to determine the relationship between circFBXO11 and miR-376a-3p as well as between miR-376a-3p and SNRPB. The si-NC, si-circFBXO11, miR-NC, miR-376a-3p, si-SNRPB, si-circFBXO11+anti-miR-NC, si-circFBXO11+anti-miR-376a-3p, si-circFBXO11+pcDNA-NC, si-circFBXO11+pcDNA-SNRPB were transfected into gastric cancer SNU-1 cells, respectively. CCK-8 assay, Flow cytometry and WB assay were used to detect cell proliferation activity, apoptosis rate and protein expressions of SNRPB, cyclin D1 and C-caspase-3, respectively. Results: Compared with para-cancerous tissues, the expression level of circFBXO11 and the positive rate of SNRPB protein in gastric cancer tissues were significantly increased (all P<0.01), while the expression of miR-376a-3p was significantly decreased (P<0.01). Compared with GES1 cells, the expressions of circFBXO11 and SNRPB were significantly increased, while the expression of miR-376a-3p was significantly decreased (all P<0.01) in gastric cancer cells. circFBXO11 negatively regulated miR-376a-3p expression, and miR-376a-3p negatively regulated SNRPB expression. After inhibiting the expression of circFBXO11 or over-expressing miR-376a-3p or suppressing the expression of SNRPB, the proliferation viability of SNU-1 cells was decreased, and the apoptosis rate was increased (P<0.01). Either inhibiting miR-376a-3p or over-expressing SNRPB could partially reverse the effect of circFBXO11 suppression on proliferation and apoptosis of SNU-1 cells (all P<0.01). Conclusion: circFBXO11 is highly expressed in gastric cancer tissues. Inhibiting circFBXO11 inhibits the proliferation and induces apoptosis of gastric cancer cells, and the mechanism is related to the regulation of miR-376A-3p/SNRPB pathway.
- Full text:20210409.pdf
