Effect of BRCA1 on proliferation, migration and invasion of non-small cell lung cancer H1650 cells via Wnt/β-catenin pathway
DOI：10.3872/j.issn.1007-385x.2021.04.006
- VernacularTitle:BRCA1通过Wnt/β-catenin通路对非小细胞肺癌H1650细胞增殖、迁移和侵袭的影响
- Author:
YAO Jie1
1
;
WEI Yaping1,
1
;
LIU Han1
1
;
LI Hailian1
1
;
CHEN Qian2
2
Author Information
1. 1. Department of Oncology, Fuxing Hospital of Capital Medical University, Beijing 100038, China
2. 2. Sozhen [Beijing] Medical Technology Co., Ltd., Beijing 100176, China
- Publication Type:Journal Article
- Keywords:
breast cancer susceptibility gene 1 (BRCA1);
Wnt/β-catenin pathway;
non-small cell lung cancer (NSCLC);
H1650 cell;
proliferation;
migration;
invasion
- From:
Chinese Journal of Cancer Biotherapy
2021;28(4):353-358
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the effect of breast cancer susceptibility gene 1 (BRCA1) on the proliferation, migration and invasion of non-small cell lung cancer (NSCLC) H1650 cells through Wnt/β-catenin pathway. Methods: WB and qPCR were used to detect the mRNA and protein expressions of BRCA1 in NSCLC A549, H1299, H1650 cells and normal lung epithelial BEAS-2B cell. A stable BRCA1 over-expression cell line (LV-BRCA1) was constructed in H1650 cells, and blank control group (NC), negative control group (LV-BRCA1-NC), experimental group (LV-BRCA1) and inhibitor group (LV-BRCA1+XAV-939) were set up. The proliferative activity of cells in each group was detected by MTT assay, the migration ability of cells was detected by scratch test, the invasive ability of cells was detected by Transwell method, and the protein expression levels of BRCA1, cyclin D1, β-catenin, c-Myc and Cox2 were detected by WB. Results: The mRNA and protein expression levels of BRCA1 in NSCLC cells were significantly higher than those in BEAS-2B cells (all P<0.01). Up-regulation of BRCA1 expression in H1650 cells could significantly enhance cell proliferation, migration and invasion (P<0.05 or P<0.01), and increase the protein expressions of cyclin D1, β-catenin, c-Myc, Cox2 and c-Jun (P<0.05 or P<0.01). β-catenin inhibitor XAV-939 significantly down-regulated the proliferation, migration and invasion ability of H1650 cells over-expressing BRCA1, and decreased the protein expressions of cyclin D1, β-catenin, c-Myc, Cox2 and c-Jun (P<0.05 or P<0.01). Conclusion: BRCA1 can promote the proliferation, migration and invasion of NSCLC H1650 cells by activating Wnt/β-catenin pathway, and it is expected to be a potential diagnostic biomarker and treatment target for NSCLC.
- Full text:20210406.pdf
