Effect of light at night on liver metabolism in miceby untargeted metabolomics
10.19485/j.cnki.issn2096-5087.2021.02.006
- Author:
ZHANG Rong
;
LIU Chun Hua
;
HU Shuang
;
LI Peng Xiang
;
WEI Shou Gang
- Publication Type:Journal Article
- Keywords:
light at night;
metabolomics;
differential metabolites;
metabolic pathway
- From:
Journal of Preventive Medicine
2021;33(2):130-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the changes of liver metabolism in mice exposed to artificial light at night.
Methods:Healthy male C57BL/6J mice were randomly divided into the light at night group and the control group, with 8 mice in each group. The daily light/dark cycle was 12/12 hours in the control group, and 24/0 hours in the light at night group for 10 consecutive days. The hepatic metabolite profiles of the two groups of mice were detected by high performance liquid chromatography tandem mass spectrometry. The modelling was assessed by combining principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis ( OPLS-DA ) , The changes of metabolites in the two groups were compared through KEGG database.
Results:Compared with the control group, 9 different metabolites were detected in the light at night group, among which the down-regulated metabolites were glycine-betaine, glutathione, tyrosine, betaine, lysine, hypoxanthine, histidine and methionine, and the up-regulated ones were mannose-6-phosphate. The weight analysis of the metabolic pathways showed that the major influences on liver of light at night group were phenylalanine-tyrosine-tryptophan metabolism, tyrosine metabolism, fructose and mannose metabolism, cysteine and methionine metabolism and histidine metabolism.
Conclusion:The metabolism of various amino acids and sugars in light at night mice is disturbed,and the key differential metabolites are tyrosine, methionine, histidine and mannose-6-phosphate.
- Full text:夜间光暴露小鼠肝脏非靶向代谢组学研究.pdf
