Developmental toxicity of 2,4-dinitrochlorobenzene in zebrafish embryos
10.19485/j.cnki.issn2096-5087.2021.02.004
- Author:
LI Xue Qing
;
XING Ming Luan
;
WANG Xiao Feng
- Publication Type:Journal Article
- Keywords:
2,4-dinitrochlorobenzene;
zebrafish embryos;
lethal effects;
toxicity
- From:
Journal of Preventive Medicine
2021;33(2):121-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the developmental toxicity of 2,4-dinitrochlorobenzene in zebrafish embryos.
Methods:AB wild-type male and female zebrafish were selected to mate and spawn, then the eggs were cultured with Holt buffer solution. Six dose groups ( 0.4, 0.8, 1.0, 1.2, 1.6, 2.0 μg/mL ), a solvent control group and a cosolvent control group, were set up with 20 embryos each. Malformations and death of embryos were observed at 48, 72 and 96 hpf ( hours post fertilization ), the mortality and 50% lethal concentration ( LC50 ) were also calculated.
Results:At 48, 72 and 96 hpf, the LC50 of 2,4-dinitrochlorobenzene on zebrafish embryos were 1.668, 1.043 and 0.895 μg/mL, respectively, with a downward trend. After 72 hpf, when the concentration reached 2.0 μg/mL, all the zebrafish died. In the range of 0.4-2.0 μg/mL, the mortality of zebrafish at 48, 72 and 96 hpf increased with the increase of 2,4-dinitrochlorobenzene concentration ( all P<0.05 ); the malformation rate of zebrafish embryos at 48 hpf increased with the increase of 2,4-dinitrochlorobenzene concentration ( P<0.05 ). Zebrafish embryos exposed to 2,4-dinitrochlorobenzene led to yolk sac edema, pericardial edema and spinal curvature.
Conclusion:2,4-dinitrochlorobenzene can affect the development of zebrafish embryos, which will lead to lethal and teratogenic effects.
- Full text:2,4-二硝基氯苯对斑马鱼胚胎发育的毒性作用研究.pdf
