Immunomodulatory effect of beta-glucan derived from Saccharomyces cerevisiae strains.
- Author:
Young Oh LEE
1
;
Myeong Su SHIN
;
Wan Kyu LEE
Author Information
1. Laboratory of Veterinary Bacteriology, College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea. wklee@cbu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Saccharomyces cerevisiae;
beta-glucan;
macrophage;
NO;
cytokine
- MeSH:
beta-Glucans;
Cytokines;
Macrophages;
Nitric Oxide;
Saccharomyces;
Saccharomyces cerevisiae;
Sprains and Strains;
Tumor Necrosis Factor-alpha;
Yeasts
- From:Journal of Biomedical Research
2013;14(1):23-27
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The aim of this study was to evaluate immunopotentiating activities of beta-glucan derived from Saccharomyces (S.) cerevisiae and to select new strains having possibility as an immune-enhancing substance. We examined SB20 strains derived from commercial product as a control, and extracted beta-glucans from the four strains of S. cerevisiae. RAW264.7 macrophages were treated with heat-killed yeasts, beta-glucans, and lipopolysaccharide (LPS). The production of nitric oxide (NO) and cytokines such as TNF-alpha and IL-1beta were then quantified. When macrophages were induced directly by in vitro addition of beta-glucan, little production of NO and IL-1beta was observed. When pretreated with strong stimulants, i.e., LPS, most yeasts showed down-modulation of NO and IL-1beta production. However, TNF-alpha secretion was triggered by beta-glucans and even more increased by the mixture effect of LPS and beta-glucans. In particular, S6 strain induced TNF-alpha secretion more than other strains. Therefore, we can conclude that the S6 strain has possibility as an immune-enhancing substance.
