Potential biomarkers in NASH-induced liver cirrhosis with hepatocellular carcinoma: A preliminary work on roles of exosomal miR-182, miR-301a, and miR-373
- Author:
Allia Najmie Muhammad Yusuf
1
Author Information
1. Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia
- Collective Name:Raja Affendi Raja Ali; Khairul Najmi Muhammad Nawawi; Norfilza Mohd Mokhtar
- Publication Type:Journal Article
- Keywords:
Exosomes;
miR-182 microRNA;
miR-301 microRNA;
miR-373 microRNA;
hepatocellular carcinoma
- From:The Malaysian Journal of Pathology
2020;42(3):377-384
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Recent studies have published the roles of exosomal miRNAs in the pathogenesis of
various type of malignancies and can be developed as potential biomarkers for diagnostic, prognostic
and therapeutic purposes. The aim of this study was to identify the expression level of selected
miRNAs (miR-182, miR-301a, and miR-373) in exosomes of the serum and ascitic fluid in patients
with non-alcoholic steatohepatitis (NASH)-related liver cirrhosis with or without hepatocellular
carcinoma (HCC). Materials and Methods: A literature search was performed to identify potential
miRNAs involved in the pathogenesis of HCC. Unpaired serum and ascitic fluid were obtained from
52 patients with NASH related liver cirrhosis (n=26 for each group of with and without HCC).
Exosomal miRNA was isolated from all samples. Expression levels of miR-182, miR-301a and miR373 were determined using quantitative real-time PCR. Results: Serum-derived exosomal mir-182,
miR-301a and miR-373 were significantly up-regulated with fold change of 1.77, 2.52, and 1.67
(p< 0.05) respectively in NASH-induced liver cirrhosis with HCC as compared to NASH-induced
liver cirrhosis without HCC. We identified the expression levels of ascitic fluid-derived exosomal
mir-182, miR-301a, and miR-373 were significantly up-regulated with fold change of 1.6, 1.94 and
2.13 respectively in NASH-induced liver cirrhosis with HCC as compared to NASH-induced liver
cirrhosis without HCC (p <0.05). There was poor correlation expression of all the selected exosomal
miRNA between serum- and ascitic fluid-derived in HCC group. Conclusions: This preliminary data
showed significant increase in the expression levels of exosomal miR-182, miR-301a and miR373 in both serum and ascetic fluid suggesting the possible roles of these miRNAs as circulating
biomarkers for NASH-induced liver cirrhosis with hepatocellular carcinoma
- Full text:5.2020my01084.pdf
