Identification of the bacteria strain and its antibiotic resistance in an epidemic of cholera
10.19428/j.cnki.sjpm.2021.19226
- VernacularTitle:一起霍乱疫情的病原检测及药敏分析
- Author:
Xiao-hong ZHOU
1
;
Pei-hua XU
;
Yan-jing NI
;
Jian CHI
;
Xiao-wei ZHU
Author Information
1. Haiyan Center for Disease Control and Prevention, Haiyan, Zhejiang 314300, China
- Publication Type:Research Article
- Keywords:
cholera;
O139 serogroup;
etiological pathogen detection;
antibiotic resistance test
- From:
Shanghai Journal of Preventive Medicine
2021;33(2):128-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The present study was conducted to identify the Vibrio cholera type and to analyze its antibiotic resistance in an epidemic of cholera in Haiyan County in 2018, which would provide the references for prevention and control of cholera. Methods Stool samples of the patient and his close contacts as well as the food and environmental samples were collected for identification of the type of Vibrio cholerae and the toxin gene. The resistance of identified Vibrio cholerae to 20 different common antibiotics were tested. Results A total of 176 samples were collected, including 101 stool samples from the case and his close contacts, 35 environmental samples and 40 food samples. Among those samples, only one strain of V. cholerae, O139, was isolated from the patient's first feces sample. It was detected as a toxin gene of ctxA positive by real-time fluorescence PCR. Antibiotic resistance test showed that the strain was sensitive to norfloxacin, levofloxacin, ciprofloxacin, cefotaxime, cephalothin, ampicillin, and amoxicillin. It was 100% resistant to tetracycline, doxycycline, neomycin, kanamycin, streptomycin, and rifampicin. Conclusion V. cholerae O139 strain with ctxA is detected in an epidemic of cholera. Norfloxacin, levofluoxacin and some other antibiotics could be used for clinical treatment and prevention. It should pay attention to this strain of V. cholera regarding the multiple drug resistance and the change of antibiotic resistance.
