Expression of ASBT and ASGPR mediated receptors for oral liver-targeting preparations in a rat model of hepatic alveolar echinococcosis
DOI:10.3969/j.issn.1001-5256.2021.04.024
- VernacularTitle:口服肝靶向制剂介导受体ASBT和ASGPR在肝泡型包虫病大鼠模型体内的表达分析
- Author:
Ruixue GAO
1
;
Chunhui HU
;
Fabin ZHANG
;
Pan GAO
;
Xuehui GAN
;
Yaogang ZHANG
;
Bofan JIANG
Author Information
1. Medical College of Qinghai University, Xining 810001, China
- Publication Type:Research Article
- Keywords:
Echinococcosis, Hepatic;
Molecular Targeted Therapy;
Apical Sodium-Dependent Bile Salt Transporter;
Asialoglycoprotein Receptor;
Rats, Sprague-Dawley
- From:
Journal of Clinical Hepatology
2021;37(4):846-851
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the feasibility of apical sodium-dependent bile salt transporter (ASBT) and asialoglycoprotein receptor (ASGPR) in the design of oral liver-targeting preparations for the treatment of hepatic alveolar echinococcosis (HAE) by measuring the expression of ASBT and ASGPR. MethodsA total of 18 male Sprague-Dawley rats were selected, among which 10 were used to establish a model of HAE (HAE group) and 8 were used as controls (normal group). Immunofluorescence assay, Western blotting, and quantitative real-time PCR were used to measure the expression distribution, protein expression level, and mRNA expression level of ASBT in the ileal tissue of HAE model rats and normal rats; the same methods were used to measure the expression level of ASGPR in the non-diseased liver tissue and the marginal zone of liver tissue lesion of HAE model rats and the liver tissue of normal rats. The t-test was used for comparison of normally distributed continuous data between two groups; a one-way analysis of variance was used for comparison between three groups, and the least significant difference t-test was used for comparison between two groups. ResultsThe results of immunofluorescence assay, Western blotting, and quantitative real-time PCR showed that compared with the normal group, the HAE group had significantly upregulated expression of ASBT in the ileal tissue (t=5309, 4.110, and 28.060, all P＜0.05) and a significantly higher expression level of ASGPR (the closer to the lesion, the higher the expression) (F=110666, 128.201, and 143.879, all P＜0.001). ConclusionASBT and ASGPR can be used as potential mediated receptors for oral liver-targeting preparations for HAE, which provides a theoretical basis for the design of oral liver-targeting preparations for the treatment of HAE.
