Comprehensive analysis on expression and biological role of miR-224 in hepatocellular carcinoma
DOI: 10.3872/j.issn.1007-385x.2021.02.006
- VernacularTitle:综合性分析miR-224 在肝癌中的表达及其生物学作用
- Author:
ZONG Yanhong1,
1
,
2
;
YU Fang1
1
,
2
;
LIU Yilong2
3
;
HUO Lijing1
1
,
2
;
TANG , Zhipeng1
1
,
2
;
TAN He1
1
,
2
;
TIE Yanqing1
1
,
2
Author Information
1. 1. Department of Clinical Laboratory, Hebei Provincial People'
2. s Hospital, Shijiazhuang, 050051, Hebei, China
3. 2. Medical Team, The Shijiazhuang Detachment of the Armed Police Security Team, Shijiazhuang 050051, Hebei, China
- Publication Type:Clinical Trial
- From:
Chinese Journal of Cancer Biotherapy
2021;28(2):135-142
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To analyze the expression level of miR-224 in cancer tissues and plasma of hepatocellular carcinoma (HCC)
patients, and its correlation with clinicopathological characteristics, diagnosis and prognosis of HCC patients, and to further analyze its
mechanism of action in the occurrence and development of liver cancer through bioinformatics analysis and in vitro experiments.
Methods: The expression level of miR-224 in HCC tissues and normal tissues was analyzed using large sample data from Gene
Expression Omnibus (GEO). qPCR method was used to verify the expression level of miR-224 in the tumor tissues and
corresponding adjacent tissues that surgically resected from 80 HCC patients in Hebei Provincial People ’s Hospital from January
2017 to January 2020; in addition, the miR-224 level was also examined in plasma samples from 30 HCC patients. The Kaplan-Meier
plotter database was used to analyze the correlation between the miR-224 expression and the overall survival time of HCC patients.
The biological processes and signal pathways involving miR-224 were analyzed using bioinformatics tools. Hepatocellular
carcinoma HepG2 cells were transfected with miR-224 inhibitor, and then Clone formation experiment, Transwell chamber
experiment, qPCR and WB methods were used to detect the effect of miR-224 knockdown on the proliferation and invasion of
HepG2 cells and the expression level of EMT-related molecules. Results: The results of GEO database analysis showed that the
expression level of miR-224 in HCC tissues was significantly higher than that in normal tissues. The results of clinical specimen verification showed that the expression level of miR-224 in the tumor tissues and plasma of HCC patients was significantly higher than
that in the corresponding adjacent tissues and plasma from healthy controls (all P<0.01). The expression level of miR-224 was
significantly correlated with the TNM stage, lymph node metastasis status and tumor size of HCC patients (P<0.05 or P<0.01). ROC
analysis indicated that miR-224 showed a prominent diagnostic value in liver cancer, and the increased expression level of miR-224 was
significantly related to the poor prognosis of HCC patients (P<0.05). Functional enrichment analysis revealed that miR-224 was mainly
involved in the mTOR signaling pathway, AGE-RAGE signaling pathway, Rap1 signaling pathway, Ras signaling pathway, ErbB
signaling pathway, HIF-1 signaling pathway and p53 signaling pathway and other signaling pathways related to tumor occurrence and
development. Knockdown of miR-224 could significantly inhibit the colony formation and invasion of HepG2 cells and affect the
expression of EMT-related markers (P<0.05 or P<0.01). Conclusion: miR-224 is highly expressed in HCC tissues and plasma and is
significantly related to the poor prognosis of HCC patients. Knockdown of miR-224 expression can inhibit the colony formation,
invasion and EMT process of liver cancer HepG2 cells.
- Full text:zgzlswzlzz-2021-2-135.pdf
