PDGF-BB promotes angiogenesis of human osteosarcoma through exosomes
DOI: 10.3872/j.issn.1007-385x.2021.02.005
- VernacularTitle:PDGF-BB 通过外泌体促进人骨肉瘤的血管新生
- Author:
LIU Lizhu1,
1
;
LI Chaoyi1
1
;
LIN Shiwei1
1
;
QU Ye1
1
;
TANG Qiang2
2
Author Information
1. 1. Department of Traumatic Surgery, Second Affiliated Hospital of Hainan Medical College, Haikou 570311, Hainan, China
2. 2. Key Laboratory of Tumorigenicity and Intervention of Hainan Province, Hainan Medical College, Haikou 571199, Hainan, China
- Publication Type:Journal Article
- Keywords:
osteosarcoma;
PDGF-BB;
exosome;
angiogenesis
- From:
Chinese Journal of Cancer Biotherapy
2021;28(2):128-134
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To explore whether PDGF-BB can be transmitted through exosome and verify its angiogenic function in human
osteosarcoma. Methods: Exosomes from a variety of human osteosarcoma cells were isolated. The expression of PDGF-BB in cells and
exosomes was detected by WB. Exosomes derived from osteosarcoma SJSA-1 cells were co-incubated with HUVEC, and the pattern of
exosomal PDGF-BB entering HUVEC was observed using Immunofluorescence and confocal scanning microscope. SJSA-1 cell lines
with PDGF-BB over-expression or knockdown were constructed by lentiviral infection, and the exosomes derived from transfected
SJSA-1 cells were isolated and incubated with HUVEC. Microtubule formation experiment was conducted to detect their effects on
angiogenesis; SJSA-1 cell transplanted xenograft model was established in nude mice, and the exosomes derived from SJSA-1 cells with
PDGF-BB over-expression or knockdown were infused into nude mice to observe their effects on tumor growth. Results: The exosomes
derived from osteosarcoma cells were successfully isolated, in which a large amount of PDGF-BB was confirmed. The exosomes
entered HUVEC by endocytosis. The SJSA-1 cell lines with PDGF-BB over-expression or knockdown were successfully constructed,
and the corresponding exosomes were isolated. Compared with the control group, exosomes with high PDGF-BB content significantly
promoted HUVEC angiogenesis (P < 0.01 , t=13.51) and tumor growth (P < 0.01 ), while exosomes with low PDGF-BB content
reduced the angiogenesis ability of HUVEC (P < 0.01 , t=8.226) and inhibited tumor growth (P < 0.01 ). Conclusion: The exosomal
PDGF-BB secreted by osteosarcoma cells can be directly absorbed by HUVEC and induce tumor angiogenesis, further promoting the
growth of osteosarcoma.
- Full text:zgzlswzlzz-2021-2-128.pdf
