Prevalence and drug resistance of nontuberculous mycobacteriain Zhejiang Province
10.19485/j.cnki.issn2096-5087.2021.01.002
- Author:
ZHU Ye Lei
;
PAN Ai Zhen
;
ZHOU Lin
;
LIU Zheng Wei
;
ZHANG Ming Wu
;
WU Kun Yang
;
WANG Xiao Meng
;
WU Bei Bei
- Publication Type:Journal Article
- Keywords:
nontuberculous mycobacteria;
species identification;
drug resistance
- From:
Journal of Preventive Medicine
2021;33(1):6-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To learn the prevalence and drug resistance of nontuberculous mycobacteria ( NTM ) in Zhejiang Province, so as to provide evidence for NTM prevention and control.
Methods:A total of 2 878 clinical mycobacterium isolates in Zhejiang Province were collected from the drug resistance surveillance in 2008-2009, 2013-2014 and 2018-2019, PNB/TCH growth tests were used to preliminarily identify the NTM in these mycobacterium-positive isolates. 16SrRNA, rpoB, ITS and hsp65 gene sequencing analysis were used to confirm strains initially identified as NTM. Proportional method was applied to detect drug susceptibility of NTM isolates.
Results : Finally, 135 strains were confirmed as NTM and the isolation rate was 4.69%. The isolation rates of NTM in 2008-2009, 2013-2014 and 2018-2019 were 1.85%, 4.56% and 7.84%, respectively, with an increasing trend ( P<0.05 ). Thirteen species were identified and the top two species were M. intracellulare ( 82, 60.74% ) and M. kansassi ( 18, 13.33% ). The NTM isolates showed the highest drug resistance rate to isoniazid ( 97.78% ), followed by p-aminosalicylic acid ( 94.87% ) and streptomycin ( 94.81% ).
Conclusions:The isolation rates of NTM showed an upward trend in the drug resistance surveillance in 2008-2019,2013-2014 and 2018-2019 in Zhejiang Province. M. intracellulare and M. kansassi were the main strains isolated. The NTM isolates showed high resistance against both first and second-line antituberculosis drugs.
- Full text:浙江省非结核分枝杆菌流行状况及耐药性分析.pdf
