LC-MS/MS method for determination of salidroside in the capsule

VernacularTitle:LC-MS/MS法测定红景天胶囊中红景天苷含量的方法
Author: Huijun FANG ¹ ; Ye DUAN ¹ ; Chunli ZHANG ¹ ; Longji HUANG ¹ ; Rong WANG ¹
Author Information

1. Department of Pharmacy, No. 940 Hospital of Joint Logistics Support Force of the PLA, Lanzhou 730050, China.
Keywords: salidroside; salidroside capsule; LC-MS/MS
From: Journal of Pharmaceutical Practice 2021;39(2):157-159
CountryChina
Language:Chinese
Abstract: Objective To establish a LC-MS/MS method for the determination of salidroside in the capsule. Methods An electrospray ionization and multiple reaction detection were used to detect negative ion. Theophylline was used as standard. The detection ions of salidroside and theophylline used for quantitative analysis were m/z 299.0→119.0, and m/z 178.8→164.0, respectively. The Shim-pack XR-ODS (3.0 mm×75 mm, 2.0 μm) column was used for separation. The mobile phase was acetonitrile: 5 mmol/L ammonium acetate solution (90∶10, V/V). The flow rate was 0.40 ml/min. The column temperature was 25 ℃. Results The content of salidroside was analyzed within 3 minutes. The linear range was 10–2 000 ng/ml, and the minimum detection limit was 10 ng/ml. Conclusion The method has good repeatability, high sensitivity, fast analysis speed and simple operation. It can be used as a method for the determination of salidroside in the capsule. It is suitable for the quality inspection of drugs and convenient for safe use.