Determination of 11 main components in Hanshi Yufei decoction, a COVID-19 empirical prescription by UHPLC-MS/MS
10.12206/j.issn.1006-0111.202012017
- VernacularTitle:UHPLC-MS/MS法测定新冠肺炎经验方寒湿郁肺汤中11种主成分的含量
- Author:
Lili CUI
1
,
2
;
Yong CHEN
3
;
Mengwei ZHANG
1
,
2
;
Hongxia YAN
1
,
4
;
Shouhong GAO
3
;
Wansheng CHEN
3
Author Information
1. Department of Pharmacy, Changzheng Hospital Affiliated to Navel Medical University, Shanghai 200003, China
2. Yichun University, Yichun 336000, China.
3. Department of Pharmacy, Changzheng Hospital Affiliated to Navel Medical University, Shanghai 200003, China.
4. Yunnan University of Chinese Medicine, Kunming 650000, China.
- Keywords:
UHPLC-MS/MS;
content determination;
COVID-19;
Hanshi Yufei decoction
- From:
Journal of Pharmaceutical Practice
2021;39(2):148-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method to determine 11 main components in Hanshi yufei decoction. Methods The method adopted UHPLC-MS/MS with an Agilent ZORBAX SB-C18 (3.5 μm，2.1 mm×150 mm) column. The mobile phase was consisted of 0.2% formic acid plus 10 mmol/L ammonium acetate aqueous solution(A) - acetonitrile(B) and gradient elution (0–0.6 min, 80%–40%A; 0.6–1 min, 40%–30%A; 1–4.3 min, 30%–5%A) at 0.3 ml/min. The column temperature was 40 ℃ and 11 main components including vanillic acid, magnolol, honokiol, wogonin, sophorin, 6-gingerol, citrinin, qianghuo alcohol, nobiletin, nodakenin, and hesperidin were quantified in a multiple reaction monitoring mode. The reserpine was the standard. Results The 11 main components in Hanshi Yufei decoction had a good linear relationship within their concentration range (r>0.98), and the average recovery was 93.11%～111.73%. Conclusion The UHPLC-MS/MS method established in this experiment is easy to operate and has good reproducibility, which provides a laboratory basis for the quality control of Hanshi Yufei decoction.
