Effects of upadacitinib on BV2 cells after oxygen–glucose deprivation/recovery
10.12206/j.issn.1006-0111.202012006
- VernacularTitle:乌帕替尼对氧糖剥夺/复氧后BV2细胞极化的影响
- Author:
Zhibing SONG
1
,
2
;
Qian ZHANG
1
,
2
;
Yuefan ZHANG
3
;
Yan QIU
4
;
Tiejun LI
1
,
2
Author Information
1. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
2. Department of Pharmacy, Punan Hospital of Pudong New Area, Shanghai 200125, China.
3. Department of Pharmacology, School of Pharmacy, Naval Medical University, Shanghai 200433, China.
4. People′s Hospital of Pudong New Area, Shanghai 201200, China.
- Keywords:
upadacitinib;
OGD/R;
polarization, inflammation, JAK/STAT
- From:
Journal of Pharmaceutical Practice
2021;39(2):112-117
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of upadacitinib on the polarization and inflammation of BV2 microglia after oxygen glucose deprivation/recovery (OGD/R) and to explore its mechanism of action. Methods The experiment was divided into 3 groups: control group, OGD group and upadacitinib treatment group. After BV2 cells were treated with OGD/R, MTT was used to detect cell survival rate. Wound scratch assay was used to detect the cell migration ability. qPCR was used to detect mRNA levels of M1-type polarization markers (CD11b, CD32, iNOS) and M2-type polarization markers (Arg-1, IL-10, CD206) of BV2 cells. ELISA was used to detect the levels of IL-1β, IL-6, and TNF-α in the culture medium. Western blot was used to detect the expression levels of JAK1/STAT6 pathway-related proteins. Results Upadacitinib increased the survival of BV2 cells after OGD/R (P<0.05), reduced the polarization of BV2 cells to M1 type (P<0.05). Upadacitinib significantly decreased the migration ability of BV2 cells induced by OGD/R (P<0.05), reduced the inflammatory factors secreted by BV2 cells induced by OGD/R: IL-1β, IL-6, TNF-α (P<0.05). Upadacitinib increased the survival rate of co-cultured PC12 cells (P<0.05). Upadacitinib significantly inhibited the expression levels of p-JAK1 and p-STAT6 proteins in BV2 cells activated by OGD/R induction (P<0.05). Conclusion Upadacitinib decreases polarization of BV2 induced by OGD/R to M1 type and reduces inflammation, which is related to JAK1/STAT6 pathway.
