Expression and evaluation of porcine circovirus type 2 capsid protein mediated by recombinant adenoassociated virus 8
- Author:
Shuang LI
1
;
Bo WANG
;
Shun JIANG
;
Xiaohui LAN
;
Yongbo QIAO
;
Jiaojiao NIE
;
Yuhe YIN
;
Yuhua SHI
;
Wei KONG
;
Yaming SHAN
Author Information
- Publication Type:Original Article
- From:Journal of Veterinary Science 2021;22(1):e8-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:Porcine circovirus type 2 (PCV2) is an important infectious pathogen implicated in porcine circovirus-associated diseases (PCVAD), which has caused significant economic losses in the pig industry worldwide.
Objectives:A suitable viral vector-mediated gene transfer platform for the expression of the capsid protein (Cap) is an attractive strategy.
Methods:In the present study, a recombinant adeno-associated virus 8 (rAAV8) vector was constructed to encode Cap (Cap-rAAV) in vitro and in vitro after gene transfer.
Results:The obtained results showed that Cap could be expressed in HEK293T cells and BABL/c mice. The results of lymphocytes proliferative, as well as immunoglobulin G (IgG) 2a and interferon-γ showed strong cellular immune responses induced by Cap-rAAV. The enzyme-linked immunosorbent assay titers obtained and the IgG1 and interleukin-4 levels showed that humoral immune responses were also induced by Cap-rAAV. Altogether, these results demonstrated that the rAAV8 vaccine Cap-rAAV can induce strong cellular and humoral immune responses, indicating a potential rAAV8 vaccine against PCV2.
Conclusions:The injection of rAAV8 encoding PCV2 Cap genes into muscle tissue can ensure long-term, continuous, and systemic expression.
