A Comparative Study on Aqueous Chitosan Solution and Various Submucosal Injection Fluids Using a Three-Dimensional Sensor
- Author:
Han Jo JEON
1
;
Seung JEONG
;
Hyuk Soon CHOI
;
Se Hyun JANG
;
Sang Hoon KIM
;
Seung Han KIM
;
Jae Min LEE
;
Eun Sun KIM
;
Bora KEUM
;
Yoon Tae JEEN
;
Hong Sik LEE
;
Hoon Jai CHUN
;
Jong Hoon CHUNG
;
Seong Nam KIM
Author Information
- Publication Type:Original Article
- From:Gut and Liver 2021;15(2):217-224
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background/Aims:Chitosan, a natural polymer widely used in the biomaterials field, has been proposed as a potential submucosal injection solution. The purpose of this study was to compare the performance and efficacy of aqueous chitosan solution and commercialized submucosal injection fluids using a three-dimensional sensor and to evaluate the efficacy of the measured parameters.
Methods:Normal saline (0.9% NaCl), as a control, Eleview ® (Poloxamer 188), Blue Eye TM (0.4% hyaluronic acid), and aqueous chitosan solution (2.0%) were injected into the submucosa of porcine stomachs ex vivo. The mucosal elevation height, elevated surface area, and angle of the tangent of the submucosal fluid cushion were measured using a three-dimensional sensor. The rates of change for each variable were calculated, and the correlation between parameters was analyzed. Tissue specimens were stained with hematoxylin and eosin.
Results:All variables exhibited the highest values under chitosan injection. The mucosal elevation height rate of change differed significantly between normal saline and chitosan solution (p=0.024). The elevated surface area rates of change for normal saline and Eleview® were significantly different from those for TS-905 and chitosan solution (p=0.006 and p=0.009, respectively). Further, height, area, and angle showed a positive correlation (p<0.001). A histological examination revealed an even distribution of aqueous chitosan within the submucosa without tissue damage.
Conclusions:Aqueous chitosan was superior to normal saline and Eleview® and was noninferior to TS-905. A three-dimensional sensor and the measured parameters were effective and useful for evaluating the performance of submucosal fluids.
