Cloning and expression analysis of chalcone isomerase from Aquilaria sinensis
10.16438/j.0513-4870.2020-1588
- VernacularTitle:白木香查尔酮异构酶基因的克隆鉴定与表达分析
- Author:
Ning DING
1
;
Yan HAI
1
;
Xiao-hui WANG
1
;
Peng-fei TU
1
;
Bo-wen GAO
2
;
She-po SHI
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
2. Baotou Medical College, Baotou 014060, China
- Publication Type:Research Article
- Keywords:
italic>Aquilaria sinensis;
agarwood;
chalcone isomerase;
gene cloning and function determination;
expression analysis
- From:
Acta Pharmaceutica Sinica
2021;56(2):630-638
- CountryChina
- Language:Chinese
-
Abstract:
Chalcone isomerases (CHIs) play an essential role in the biosynthesis of flavonoids important in plant self-defense. Based on the transcriptome data of Aquilaria sinensis Calli, a full-length cDNA sequence of CHI1 (termed as AsCHI1) was cloned by reverse transcription PCR. AsCHI1 contains a complete open frame (ORF) of 654 bp. The deduced protein is composed of 217 amino acids, with a predicted molecular weight of 23.11 kDa. The sequence alignment and phylogenetic analysis revealed that AsCHI1 has conserved most of the active site residues in type I CHIs, indicating a close relationship with the CHI from Gossypium hirsutum. The recombinant AsCHI1 protein was obtained by heterologous expression of AsCHI1 in E. coli BL21(DE3). The purified AsCHI1 protein exhibited CHI activity by catalyzing the production of naringenin from naringenin chalcone. Remarkably, AsCHI1 expression in A. sinensis Calli treated with various abiotic stresses including salt, mannitol, cold, and heavy metals could be markedly increased, and plant hormones such as abscisic acid (ABA), gibberellin (GA3), and salicylic acid (SA) could also increase the expression of AsCHI1, suggesting that AsCHI1 might play an important role in plant self-defense. The results expand our understanding of the biosynthesis of flavonoids in A. sinensis and give further insight into the defensive responses of A. sinensis to abiotic and biotic stresses.
