Study on Effects and Active Ingredients of Tibetan Medicine Lamiophlomis rotata against Rheumatoid Arthritis ZHANG Yanfei ，CHEN Ruixin ，YUAN Maohua ，YANG Zhizhong ，HUANG Ting ，CHEN Suxian ，JIANG Yunbin （578） Effects of Isoflavaspidic Acid PB on the Biofilm Adhesion and Gene Expression of Ergosterol Metabolism Related Enzymes in Trichophyton rubrum
- VernacularTitle:异黄绵马酸PB对红色毛癣菌生物被膜黏附及甾醇代谢相关酶基因表达的影响
- Author:
Jie HOU
1
;
Chunping TANG
1
;
Zhibin SHEN
1
;
Yanfen CHEN
1
;
Shen DING
1
;
Zhisi ZHANG
1
;
Xiaoying LI
1
;
Tao JIANG
2
,
3
Author Information
1. College of Chinese Materia Medica，Guangdong Pharmaceutical University，Guangzhou 510006，China
2. Laboratory Animal Center，Guangdong Pharmaceutical University，Guangzhou 510006，China
3. Guangzhou Key Laboratory of Construction and Application of New Drug Screening Model Systems，Guangzhou 510006，China
- Publication Type:Journal Article
- Keywords:
Isoflavaspidic acid PB;
Trichophyton
- From:
China Pharmacy
2021;32(5):584-589
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study the effect of isofla vaspidicacid PB （called PB for short ）on the biofilm adhesion and the gene expression of ergosterol metabolism related enzymes in Trichophyton rubrum . METHODS ：M38-A2 method was adopted to determine MIC of PB to T. rubrum . MTT assay was used to screen the biolfilm condition and initial adhesion period of T. rubrum . The effects of different concentrations of PB （40，80，160 µg/mL）on the adhesion duration of T. rubrum （growth control group without PB was set up ，similarly hereinafter ）were evaluated and the adhesion rate was calculated by using XTT assay ；the effects of different concentrations of PB （20，40，80 µg/mL）on the biofilm formation of T. rubrum at different initial adhesion periods （3，5，9 h）were observed and the adhesion rate was calculated by using XTT assay combined with inverted microscope ；qRT-PCR method was used to detect the effects of PB （320 µg/mL）on the mRNA expression of ergosterol metabolism related enzyme gene ERG6 and ERG11 in biofilm of T. rubrum . RESULTS ：MIC of PB to T. rubrum was 20 µg/mL. The biofilm of T. rubrum in RPMI-1640 medium containing 10％ FBS was the most metabolism activity at 6 h of initial adhesion. Compared with growth control group ，after treated with different concentrations of PB ，adhesion rate and mRNA expression of ERG6 and ERG11 in biofilm were decreased significantly （P＜0.01）. Hyphae decreased or even disappeared ，and the adhesion inhibition rate （at 5 and 9 h of initial adhesion ）increased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS ：PB can inhibit the adhesion of T. rubrum and reduce the hyphae ；the mechanism may be associated with the inhibition of the biofilm adhesion and mRNA expression of ergosterol metabolism related enzyme gene ERG6 and ERG11.
