Improvement of Quality Standard for Mongolian Medicine Artemisia sacrorum
- VernacularTitle:蒙药哈日-沙布嘎（铁杆蒿）的质量标准提升
- Author:
Xiaohong YUAN
1
;
Kejian PANG
2
;
Hui TANG
1
;
Hong ZHANG
3
;
Feng WEI
1
;
Lele LIU
1
;
Guozhen JIANG
1
Author Information
1. Pharmacy School of Shihezi University/Key Laboratory of the Ministry of Education of Xinjiang Special Plant Medicinal Resources，Xinjiang Shihezi 832000，China
2. Hetian Uygur Pharmaceutical Co.，Ltd.，Xinjiang Hetian 848200，China
3. Mongolian Medical Hospital of Bortala Mongolian Autonomous Prefecture，Xinjiang Bole 833400，China
- Publication Type:Journal Article
- Keywords:
Mongolian medicine;
Artemisia sacrorum;
Quality standard;
Microscopic identification;
TLC;
HPLC
- From:
China Pharmacy
2021;32(5):536-541
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To improve the quality standard of M ongolian med icine Artemisia sacrorum ，and to provide scientific basis for comprehensive quality evaluation. METHODS ：The appearance and microscopic characteristics of A. sacrorum were identified；scopoletin，chlorogenic acid ，caffeic acid ，scopoletin and 3，5-dicaffeoylquinic acid were identified quantitatively by TLC；the contents of above 5 components were determined by HPLC. The water content ，total ash and extract were examined. RESULTS：The stem of A. sacrorum was cylindrical ，and its surface was purple or purple-brown or cyan-brown ；the leaves were ovate or oblong-ovate ，fragrant；the flowers were yellow ，head-shaped，subglobose or hemispherical. The powder was green or yellow-green，its pollen grain had three germination ；the parenchymal cell clusters with sharp edges and numerous threaded ducts ， occasionally having marginal pitted ducts ；its wood fibers were in bundles mostly. Results of TLC showed that the spots of the same color were found in the corresponding positions of chromatogram for 5 substance control and samples. The linear range of scopoletin， chlorogenic acid ， caffeic acid ， scopolactone and 3，5-dicaffeoylquinic acid were 85.60-428.00， 10.16-101.60， 10.20-102.00，40.84-408.40 and 40.80-408.00 μg/mL（all r＞0.999 0）. RSDs of precision ，stability，repeatability tests were all less than 3.00％（n＝6）. The average recoveries were 103.07％，99.66％，98.37％，97.78％，98.40％（all RSDs ＜3.00％，n＝6）. The contents of the above-mentioned 5 compounds in 10 batches of samples were 0.36％-1.23％，0.09％-0.51％，0.04％-0.13％， 0.61％ -1.13％ ，0.12％ -1.11％ ，respectively；the average com contents of water ，total ash and water soluble extract were 6.25％，5.86％，26.50％，respectively. CONCLU SIONS：O the basis of the original quality standard of A. sacrorum ， microscopic identification，TLC identification ，content determination and examination items of water ，total ash and extract are added. The method shows good precision ，accuracy and stability ，which can provide reference for more scientific and standardized evaluation of the quality of this medicinal material.
