Applications and progress of chemical derivatization in targeted metabolomics LC-MS analysis
10.11665/j.issn.1000-5048.20210104
- VernacularTitle:化学衍生化技术在靶向代谢组学LC-MS中的应用与进展
- Author:
Xien ZHANG
1
;
Di WANG
;
Fengguo XU
Author Information
1. 中国药科大学药物质量与安全预警教育部重点实验室
- Publication Type:Journal Article
- Keywords:
derivatization;
LC-MS;
metabolomics;
quantitative analysis
- From:
Journal of China Pharmaceutical University
2021;52(1):31-37
- CountryChina
- Language:Chinese
-
Abstract:
The changes of metabolic profile are closely related to external stimulus, and the concentration of the metabolite can directly reflect the physiological or pathological states of organisms. Therefore, the quantitative detection of metabolites is necessary. However, traditional targeted metabolomic methods have such drawbacks as narrow coverage and low sensitivity. In recent years, derivatization techniques have developed rapidly in the field of metabolomics. Derivatization reagents for amine, hydroxyl, carboxyl, carbonyl, hydrosulphonyl and other groups have been used in metabolomics research. This paper introduces various derivatization reactions and their applications according to group classification and reviewes the characteristics of multi-group derivatization techniques, with a propect of their research directions and challenges.
